遗传 ›› 2006, Vol. 28 ›› Issue (10): 1233-1236.

• 研究报告 • 上一篇    下一篇

牛白细胞粘附缺陷病(BLAD)的调查

马金柱1; 崔玉东1;2; 朱战波2; 曹宏伟1; 朴范泽2   

  1. 1. 黑龙江八一农垦大学生命科技学院, 大庆 163319; 2. 黑龙江八一农垦大学动物科技学院, 大庆 163319
  • 出版日期:2006-10-01 发布日期:2006-10-01

Investigation on Bovine Leukocyte Adhesion Deficiency

MA Jin-Zhu1; CUI Yu-Dong1; 2; ZHU Zhan-Bo2; CAO Hong-Wei1; PIAO Fan-Ze2   

  1. 1. College of Life Science and Biotechnology, Heilongjiang August First Land Reclamation University, Daqing 163319, China; 2. College of Animal Science and Veterinary Medicine, Heilongjiang August First Land Reclamation University, Daqing 163319, China
  • Online:2006-10-01 Published:2006-10-01

摘要: 牛白细胞粘附缺陷病(BLAD)是一种常染色体单基因隐性遗传疾病, 病因为白细胞表面整合素CD18亚单位基因突变所致。为了解我国奶牛群中BLAD的携带和发生情况, 本研究采用RT-PCR扩增CD18基因片段后, 用TaqⅠ内切酶对扩增产物进行酶切的方法检测了1 000头1~6岁的奶牛, 结果有19头奶牛为BLAD携带牛, 即BLAD携带率为1.9%; 1头为BLAD病牛。

关键词: T-DNA, 插入突变, 遗传分析, 水稻, 侧翼序列, CD18, RT-PCR, 牛白细胞粘附缺陷病

Abstract: Bovine leukocyte adhesion deficiency ( BLAD ) is autosomal recessive disease. The pathogeny of BLAD is genic mutation of CD18-integrins on the leukocyte. In order to know the carrier and occurrence of bovine leukocyte adhesion deficiency (BLAD) among cows age from one to six years old in China, 1 000 cows were investigated by means of amplifying a CD18 gene fragment via reverse transcriptase-PCR followed by restriction digestion with TaqⅠ. Results showed that 19 cows were BLAD carriers, indicating that the BLAD carrier rate was 1.9 percent. In adition, one cow was found to have BLAD.

Key words: analysis, flanking , rice, sequence, genetic , mutation, inserted , T-DNA

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