关键词: 分子标记, 镜鲤, 群体多样性, 微卫星

Abstract:

In this paper, population genetic variability and genetic structure of five populations of an important cultivation species, mirror carp (Cyprinus carpio L.) were analyzed using 30 microsatellite loci. The observed (^H_o) and expected (^H_e) heterozygosity values, polymorphic information content (PIC) and number of effective alleles ((^A_e)) were all determined. The genetic similarity coefficient and Nei’s standard genetic distance were computed based on the allele frequencies. The Hardy-Weinberg equilibrium was checked by c2 test. Genetic differentiation and hierarchical partition of genetic

diversity were evaluated by ^F_ST and ^H_m. A dendrogram was constructed based on UPGMA methods using PHYLIP software package supported by a bootstrap value of 91.0%. Totally 7 083 fragments were procured. Their lengths were from 102 bp to 446 bp. For each locus, 1―16 alleles were amplified, adding up to 356 alleles in all the 5 populations. We found the genetic variability level was relatively high in all five populations, as shown by ^A_e = 1.07―2.30, ^H_e= 0.70―0.78 and PIC=0.69―0.75, respectively. The genetic similarity coefficients were all above 0.52, indicating their close genetic relationships. The UPGMA phylogenetic tree showed mirror carps sampled from Donggang, Fengcheng and Liaozhong were clustered into one group and the other two populations, both collected from Songpu, were grouped together. There were obvious relations between genetic distances and geographical distributions of the five populations. No fragments were amplified from some loci of EST-SSRs, which may suggest the loss of these loci in mirror carp genome or sequence divergence at the primer binding sites. These null alleles may result from selection because functional genes are under more selection pressure than non-encoding loci. Overall, population genetic variation is high for each of the five mirror carp, and the differentiations are also significant among populations.