遗传 ›› 2006, Vol. 28 ›› Issue (4): 417-421.

• 研究报告 • 上一篇    下一篇

利用放射杂交克隆板进行猪FMR1等5个基因间的连锁分析

李隐侠1;胡衍彪1;陈 杰1;徐银学4   

  1. 1.南京农业大学动物科技学院,南京 210095;2.美国华盛顿州立大学动物科技学院  华盛顿, Wa 99164-6351 美国
  • 收稿日期:2005-04-05 修回日期:2005-07-26 出版日期:2006-04-10 发布日期:2006-04-10
  • 通讯作者: 徐银学

Linkage Analysis of Five Genes in Pigs Using Radiation Hybrid Clone Panel

LI Yin-Xia1  ;HU Yan-Biao1  ;CHEN Jie1  ;LIU Hong-Lin1  ;XU Yin-Xue1  

;JIANG Zhi-Hua2    

  1. 1College of Animal Science and Technology, Nanjing Agricultural University,Nanjng 210095,China
    2Department of Animal Sciences, Washington State University, Pullman, Wa 99164-6351 ,USA
  • Received:2005-04-05 Revised:2005-07-26 Online:2006-04-10 Published:2006-04-10
  • Contact: XU Yin-Xue

摘要:

放射杂交技术在人类图谱(包括ESTs、STSs和微卫星)的构建中已证明是一种非常有效的方法。根据人类基因组X染色体上FMR1、IDSFATEBGNF8A等5个基因的信息资源,用已构建的猪/仓鼠96个放射杂交细胞系分析猪染色体该5个基因间的连锁关系。结果显示:当LOD值为4时,FMR1、IDSFATEBGNF8A都处于同一个连锁群内;当LOD值为5时,FMR1、IDS处于同一个连锁群,FATEBGN在同一个连锁群内,而F8A单独处于一个连锁群中。
 

 


 
 

关键词: 杂交体克隆板, 基因连锁分析, 放射杂交,

Abstract:

RH (radiation hybrid) has proved to be an effective method in constructing human genome maps (including ESTs, STSs and microsatellites). In this study, based on the information of five human genes (FMR1, IDS, FATE, BGN, F8A) on the X chromosome, the linkage relationship of these five genes in pigs were analyzed by a panel of 96 radiation hybrid cell lines. The results showed that FMR1, IDS, FATE, BGN, F8A were in the same linkage group, when LOD was set at 4. When LOD was set at 5, FMR1 and IDS were in the one group, FATE and BGN in the other group, and F8A was in a group by itself.

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