遗传 ›› 2008, Vol. 30 ›› Issue (10): 1312-1318.doi: 10.3724/SP.J.1005.2008.01312

• 研究报告 • 上一篇    下一篇

κ中国荷斯坦奶牛κ-酪蛋白基因第四外显子多态性与产奶性状的关联分析

鞠志花1, 2, 李秋玲1, 王洪梅1, 李建斌1, 安利国2, 杨桂文2, 仲跻峰1, 王长法1

  

  1. 1. 山东省农业科学院奶牛研究中心, 济南 250100;
    2. 山东师范大学生命科学学院, 济南 250014

  • 收稿日期:2008-02-16 修回日期:2008-02-26 出版日期:2008-10-10 发布日期:2008-10-10
  • 通讯作者: 王长法

Genetic polymorphism of k-casein gene exon 4 and its cor-relation with milk production traits in Chinese Holstein cows

JU Zhi-Hua1, 2; LI Qiu-Ling1; WANG Hong-Mei1; LI Jian-Bin1; AN Li-Guo2; YANG Gui-Wen2; ZHONG Ji-Feng1;WANG Chang-Fa1   

  1. 1. Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan 250100, China;
    2. Life Science College, Shandong Normal University, Jinan 250014, China
  • Received:2008-02-16 Revised:2008-02-26 Online:2008-10-10 Published:2008-10-10
  • Contact: WANG Chang-Fa

摘要:

摘要: 以544头中国荷斯坦奶牛为研究对象, 以k-酪蛋白基因为产奶性状的候选基因, 扩增779 bp的片段, 结合测序结果采用PCR-RFLP方法来检测k-酪蛋白基因3个位点的多态性。结果在exon 4的第10 891 bp、10 927 bp和10 988 bp处分别发生了T/C、C/A错义突变和G/A同义突变, 据此分别选择了TaqⅠ、HindⅢ、 PstⅠ等 3种限制性内切酶检测了其多态性。发现3个位点的A、B等位基因在群体中都有分布, 且处于低度多态; A 和B 等位基因的频率分别为86.03%和13.97%; AA, ABBB基因型频率分别为73.71%, 24.63%和1.66%; c2适合性检验表明, 该群体在这3个位点的突变达到Hardy-Weinberg平衡状态(P > 0.05); BB和AB基因型个体乳脂率显著高于AA基因型个体(P<0.05), AB基因型个体脂蛋白比显著高于AA基因型个体(P < 0.05), 但不同基因型对产奶量和乳蛋白率没有显著影响; 3个位点的酶切多态性在所研究群体中是紧密连锁的。说明在中国荷斯坦奶牛群体中, κ-酪蛋白B等位基因可作为改良奶牛乳脂率性状的分子遗传标记。

关键词: κ-酪蛋白基因, 荷斯坦奶牛, PCR-RFLP, 多态性, 产奶性状

Abstract:

Abstract: K-casein gene was regarded as a candidate gene for milk production traits of cows. In this study, a 779 bp fragment of k-casein gene of Chinese Holstein was amplified by polymerase chain reaction (PCR), the polymorphisms of three loci of k-casein gene were detected by PCR-RFLP with restriction endonuclease TaqⅠ, HindⅢ, PstⅠ. After sequencing, T/C single nucleotide polymorphism (SNP) was identified at nucleotide 10 891,C/A SNP was identified at nucleotide 10 927 and G/A SNP was identified at nucleotide 10 988 in exon4 of k-casein gene. Both alleles (A and B) of three loci were found in the population that showed low polymorphism. The gene frequencies of A and B were 86.03% and 13.97%, respectively. The genotype frequencies of AA, AB, and BB were 73.71%, 24.63%, and 1.66%, respectively. Statistical results of c2 test indicated that three polymorphism sites in the population fitted with Hardy-Weinberg equilibrium (P > 0.05). Meanwhile, the effect of polymorphism of κ-casein gene on milk production traits was analyzed. The results indicated that in the three loci, the different genotype of k-casein gene had no significant influence on milk yield and milk protein percent (P > 0.05). The cows with genotypes BB and AB showed higher milk fat percent than those with genotype AA ( P < 0.05 ) ; with genotype AB showed higher fat protein ratio than those with genotype AA ( P < 0.05 ). The polymorphism of the three loci in the experimental population is closely linked. The conclusion is that k-casein B allele can be used as the molecular genetic markers of modifying milk fat percent in Chinese Holstein cows.