遗传 ›› 2008, Vol. 30 ›› Issue (10): 1356-1362.doi: 10.3724/SP.J.1005.2008.01356

• 研究报告 • 上一篇    下一篇

普通小麦-纤毛鹅观草染色体异附加系的分子标记鉴定

孔令娜; 李巧; 王海燕; 曹爱忠; 陈佩度; 王秀娥   

  1. 南京农业大学作物遗传与种质创新国家重点实验室细胞遗传研究所, 南京 210095
  • 收稿日期:2008-01-15 修回日期:2008-03-12 出版日期:2008-10-10 发布日期:2008-10-10
  • 通讯作者: 王秀娥

Molecular marker analysis of wheat-Roegneria ciliaris additions lines

KONG Ling-Na; LI Qiao; WANG Hai-Yan; CAO Ai-Zhong; CHEN Pei-Du; WANG Xiu-E   

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2008-01-15 Revised:2008-03-12 Online:2008-10-10 Published:2008-10-10
  • Contact: WANG Xiu-E xiu-e

摘要: 随机选取定位于小麦和大麦7个部分同源群上的135对EST、27对STS和253对SSR引物对24个可能的普通小麦-纤毛鹅观草二体异附加系的基因组DNA进行扩增。结果表明, 55对引物在亲本普通小麦中国春、Inayama Komugi、纤毛鹅观草和Inayama Komugi-纤毛鹅观草双二倍体间有多态性扩增, 其中31对引物可以在异附加系中扩增到纤毛鹅观草特异条带。根据PCR扩增结果, 异附加系07K02、07K06、07K39、07K201、07K202、07K255和07K256所添加的纤毛鹅观草染色体归属小麦第1部分同源群; 07K07、07K08、07K09、07K11、07K14和07K17所添加的纤毛鹅观草染色体归属小麦第2部分同源群; 07K15、07K16、07K21和07K47所添加的纤毛鹅观草染色体归属小麦第6部分同源群。

关键词: 部分同源群, 异附加系, 纤毛鹅观草, 小麦, EST, STS

Abstract: A total of 135 EST, 27 STS, and 253 SSR primer pairs located in 7 homoeologous groups of wheat and barly were used for amplification of 24 possible Wheat-R. ciliaris disomic addition lines. Fifty-five primer pairs could amplified polymorphic bands between common wheat variety “Inayama Komugi” and the Inayama Komugi- R. ciliaris amphiploid, and 31 of the 55 could amplify the specific bands of R. ciliaris in the addition lines. According to the PCR (polymerase Chain Reaction) results, the added R. ciliaris chromosomes in lines 07K02, 07K06, 07K39, 07K201, 07K202, 07K255, and 07K256 belong to homoeologous group 1 of wheat; the added R. ciliaris chromosomes in lines 07K07, 07K08, 07K09, 07K11, 07K14, and 07K17 belong to homoeologous group 2 of wheat; the added R. ciliaris chromosomes in lines 07K15, 07K16, 07K21, and 07K47 belong to homoeologous group 6 of wheat

Key words: Triticum aestivum L., Roegneria ciliaris (Trin) Nevski, addition line, homoeologous group, EST, STS