遗传 ›› 2008, Vol. 30 ›› Issue (5): 583-589.doi: 10.3724/SP.J.1005.2008.00583

• 研究报告 • 上一篇    下一篇

辽宁满族11个Y-STR基因座多态性及遗传关系的分析

石美森1, 百茹峰1, 张金生2, 于晓军3   

  1. 1. 证据科学教育部重点, 实验室 (中国政法大学), 北京 100088;
    2. 辽宁省岫岩满族自治县卫生局公共卫生科, 岫岩 114300;
    3. 汕头大学医学院法医学教研室, 广东汕头 515041

  • 收稿日期:2007-10-23 修回日期:2008-01-14 出版日期:2008-05-10 发布日期:2008-05-10
  • 通讯作者: 石美森

Polymorphism analysis of 11 Y-STR loci in Manchu population and genetic relationships with 16 populations

SHI Mei-Sen1, BAI Ru-Feng1, Zhang Jin-Sheng2, YU Xiao-Jun3   

  1. 1. Key Laboratory of Evidence Science (China University of Political Science and Law), Ministry of Education, Beijing 100088, China;
    2. Bureau for Health Inspection and Supervision, Xiuyan Manchu Autonomous County, Xiuyan, Liaoning Province 114300, China;
    3. Department of Forensic Medicine, Shantou University Medical College, Shantou, Guangdong Province 515041, China
  • Received:2007-10-23 Revised:2008-01-14 Online:2008-05-10 Published:2008-05-10
  • Contact: SHI Mei-Sen

摘要:

调查了辽宁满族群体11个Y-STR基因座的多态性分布, 探讨其群体遗传学及法医学应用价值。应用Powerplex Y System荧光标记复合扩增系统检测203名满族无关男性个体的11个Y-STR基因座, 用ABI310遗传分析仪进行基因分型, 计算等位基因和单倍型频率, 并结合已公开发表的国内其他16个群体相应基因座的遗传学资料, 分析其遗传距离和聚类关系。满族个体中共检出189种单倍型, 单倍型频率多样性0.9991, 基因多样性GD值在0.4594(DYS391)~0.9258(DYS385a/b); 从遗传距离分析发现, 满族和东北汉族的遗传距离最小(0.0015), 与维吾尔族的遗传距离最大(0.1485)。结果表明, 11个Y-STR基因座在满族群体中具有较好的遗传多态性, 适用于当地区的法医学应用。与其他民族群体遗传多样性的研究, 对了解满族的起源、迁移以及相互关系有重要意义。

关键词: Y染色体, 短串联重复序列, 遗传多态性, 单倍型, 满族

Abstract:

To investigate the genetic polymorphisms of 11 Y-chromosomal short tandem repeats(STR) loci in Manchu population, and to evaluate their forensic application values and genetic relationships with 16 other populations of China, 11 Y-STR loci in 203 unrelated Manchu individuals in Liaoning were amplified with PowerPlex Y System, and the PCR products were analyzed by 310 Genetic Analyzer. Cluster analysis and phylogenic trees were applied to show the genetic distance among the populations. As a result, a total of 189 haplotypes were identified and the overall haplotypes diversity for the 11 Y-STR loci was 0.9991. The gene diversity values (GD) for each locus ranged from 0.4594 (DYS391) to 0.9258 (DYS385a/b). Comparing with 16 populations, genetic distance between Manchu and Northeast Han population was the smallest(0.0015), while genetic distance between Man and Uygur ethnic the largest(0.1485). The phylogenetic tree was similar to the results of clustering analysis. The genetic distribution of 11 Y-STR loci in Liaoning Manchu population showed favorable polymorphisms, and was thus suitable for forensic identification and paternity testing in local area. The study of genetic diversity among different populations is useful for the research of their origins, migrations and their relationships.