关键词: 鸭, 前胰岛素原基因, SNPs

Abstract:

Genetic polymorphisms of exon 2 and partial intron of preproinsulin gene were studied in Peking duck and Cherry Valley duck by PCR-SSCP and DNA sequencing technologies. Two single nucleotide mutations, T179C and C195T, were found, respectively. χ2 test reflects that the tested population of Peking duck and Cherry Valley duck were in the Hardy-Weinberg equilibrium state (P>0.05). The relationships between SNPs and the traits of carcasses were analyzed by the least square analysis, which showed that the genotype BB in three lines of Peking duck was significantly higher in carcass weight, carcass net weight and breast muscle weight than AA and BB (P<0.01) and significantly higher in leg muscle weight and sebum weight than AB (P<0.01). In addition, the genotype AA was significantly greater than AB in sebum rate (P<0.01) and carcass net weight (P<0.05), respectively. However, for Cherry Valley duck, only the genotype AB was significantly higher than AA in sebum weight and abdomen fat weight (P<0.05). The results indicated that there was significant correlation between the genetic polymorphisms of preproinsulin gene and the traits of duck carcasses and the allele B was valuable for increasing the carcass weight and breast muscle weight.