转反义蜡质基因水稻亲本后代性状分析

doi:10.3724/SP.J.1005.2008.01195

遗传 ›› 2008, Vol. 30 ›› Issue (9): 1195-1200.doi: 10.3724/SP.J.1005.2008.01195

转反义蜡质基因水稻亲本后代性状分析

刘清¹, 吴顺¹, 沈革志², 彭琼¹, 刘桃李¹, 程鹏¹, 萧浪涛¹

1. 湖南农业大学湖南省植物激素与生长发育重点实验室, 长沙 410128;
2. 上海市农业科学院作物研究所, 上海 201106

收稿日期:2007-11-22 修回日期:2008-02-15 出版日期:2008-09-10 发布日期:2008-09-10
通讯作者: 萧浪涛

Analysis on the characters of progenies of the rice transformed with anti-sense waxy gene

LIU Qing¹, WU Shun¹, SHEN Ge-Zhi², PENG Qiong¹, LIU Tao-Li¹, CHENG Peng¹, XIAO Lang-Tao¹

1. Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China; 2. Crop Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China

Received:2007-11-22 Revised:2008-02-15 Online:2008-09-10 Published:2008-09-10
Contact: XIAO Lang-Tao

摘要/Abstract

摘要：

以单质粒转化的粳稻广粳一号和双质粒共转化的籼稻01早5202所获得的转反义蜡质基因水稻后代为供试材料, 通过潮霉素抗性、PCR检测等手段分析了外源基因的遗传分离特性, 同时, 还分析了转基因材料的直链淀粉含量、waxy蛋白含量的变化特性。结果表明, 无论是采用标记基因(hpt)与目的基因(Anti-sense waxy)连锁的单质粒转化, 还是采用双质粒共转化, 其供试的后代植株材料都发生了外源基因分离现象, 且转基因植株材料的直链淀粉含量都有所下降, 有些单株的直链淀粉含量已降至10%(质量百分比)以下, 远低于对照(其直链淀粉含量为22.04%); SDS-PAGE检测结果显示, 供试的转基因材料的waxy蛋白的含量与对应的直链淀粉含量呈正相关性。

关键词: 反义蜡质基因, 水稻, waxy蛋白, 分离, 直链淀粉

Abstract:

The segregation of exogenous genes was studied by hygromycin-resistant and PCR experiments in the transgenic rice (Oryza sativa L. subsp. japonica and indica) with anti-sense waxy gene, meanwhile the change of amylose and waxy protein contents in progenies of transgenic rice was analyzed. The results showed no matter the rice Guangjing No.1(O. Sativa L. subsp. japonica) were transformed by p13w4 plasmid carrying anti-sense waxy gene and hygromycin-resistant gene, or in the rice 01Z5202 (O. sativa L. subsp. indica) were co-transformed by p13w8 plasmid carrying anti-sense waxy gene and p1300 plasmid carrying hygromycin-resistant gene, the target gene(s) had been segregated in the progenies; the content of amylose of the transgenic plants was lower than those in non-transgenic ones, and the content of amylose in some of transgenic plants was less than 10.0% (of the weight of grain), which was much lower than those in the control (about 22.04%); and the analysis with SDS-PAGE showed the content of the waxy protein are positively correlated with the con-tent of amylose in the tested transgenic rice materials.

刘清，吴顺，沈革志，彭琼，刘桃李，程鹏，萧浪涛 . 转反义蜡质基因水稻亲本后代性状分析[J]. 遗传, 2008, 30(9): 1195-1200.

LIU Qing, WU Shun, SHEN Ge-Zhi, PENG Qiong, LIU Tao-Li, CHENG Peng, XIAO Lang-Tao. Analysis on the characters of progenies of the rice transformed with anti-sense waxy gene[J]. HEREDITAS, 2008, 30(9): 1195-1200.

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转反义蜡质基因水稻亲本后代性状分析

Analysis on the characters of progenies of the rice transformed with anti-sense waxy gene

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