荷斯坦牛HSP70-1基因遗传多态性与乳腺炎抗性关系

doi:10.3724/SP.J.1005.2009.00169

遗传 ›› 2009, Vol. 31 ›› Issue (2): 169-174.doi: 10.3724/SP.J.1005.2009.00169

荷斯坦牛HSP70-1基因遗传多态性与乳腺炎抗性关系

程维杰^{1, 2},李秋玲¹,王长法¹,王洪梅¹,李建斌¹,孙延鸣²,仲跻峰¹

1. 山东省农业科学院奶牛研究中心, 济南 250100;
2. 新疆石河子大学动物科技学院, 石河子 832000

收稿日期:2008-04-17 修回日期:2008-06-27 出版日期:2009-02-10 发布日期:2009-02-10
通讯作者: 仲跻峰;孙延鸣

Research on Polymorphism of HSP70-1 gene in Holstein and the Relationship between Different Genotypes and Resistance to Dairy Mastitis

CHENG Wei-Jie^{1, 2}, LI Qiu-Ling¹, WANG Chang-Fa¹, WANG Hong-Mei¹, LI Jian-Bin¹, SUN Yan-Ming², ZHONG Ji-Feng¹

1. Dairy Cattle Rearch Center, Shandong Academy of Agricultural Science, Ji’nan 250100, China;
2. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China

Received:2008-04-17 Revised:2008-06-27 Online:2009-02-10 Published:2009-02-10
Contact: Cheng Weijie

摘要/Abstract

摘要： 以253头中国荷斯坦奶牛为研究对象, 检测HSP70-1基因的多态性, 并分析其多态性与中国荷斯坦牛体细胞评分(Somatic cell score, SCS)的相关性。首先以PCR-SSCP法寻找HSP70-1基因编码区的突变, 并通过测序确定突变的类型, 根据突变类型寻找合适的内切酶, 最终采用PCR-RFLP方法鉴定实验牛基因型; 然后分析基因多态性与中国荷斯坦牛SCS的相关性。结果表明HSP70-1基因的1 623 bp处产生G→A→C突变, 2 409 bp处产生G→A突变, 两位点都是沉默突变, 未引起氨基酸序列的改变; 经χ2 适合性检验, 中国荷斯坦牛在两个位点均未达到Hardy-Weinberg平衡状态; 同时, 群体基因座不同基因型与SCS相关分析的结果表明, 2409位点基因型与SCS相关性不显著(P>0.05), 1623位点基因型与SCS相关性显著(P<0.05), CC型SCS显著低于AG、GG型(P<0.05), CC基因型为乳腺炎抗性基因型。在中国荷斯坦奶牛群体中, HSP70-1基因CC基因型可作为改良奶牛乳腺炎抗性性状的分子遗传标记。

关键词: 单链构象多态性, 体细胞评分, 乳腺炎抗性, 限制性片段长度多态性, 热休克蛋白70

Abstract: he polymorphisms of HSP70-1 gene in 253 Chinese Holstein dairy cows were studied, and the association between the polymorphisms and somatic cell score (SCS) were analyzed. PCR-SSCP, PCR-RFLP and DNA sequencing were used to investigate mutations in the coding region of HSP70-1 gene. The G→A→C mutation at 1 623 bp and G→A mutation at 2 409 bp were found and both of them were silence mutations that caused no alteration in amino acid sequence. Chi-square test showed both loci weren′t at Hardy-Weinberg disequilibrium in Chinese Holstein. In the meanwhile, the association of 2 409 locus and SCS was not significant. However, the polymorphism at 1623 locus affected SCS signifi-cantly (P<0.05). The SCS of genotype CC was significantly lower than that of genotype AG and GG (P<0.05), so genotype CC was mastitis resistant. These results suggest that genotype CC of HSP70-1 gene may be used as a molecular and genetic marker to improve the phenotype of anti-mastitis in Chinese Holstein dairy cows.

程维杰，李秋玲，王长法，王洪梅，李建斌，孙延鸣，仲跻峰. 荷斯坦牛HSP70-1基因遗传多态性与乳腺炎抗性关系[J]. 遗传, 2009, 31(2): 169-174.

[1]	王洪梅，孔振兴，王长法，黄金明，李秋玲，侯明海，李建斌，仲跻峰. 奶牛乳铁蛋白基因5′侧翼区遗传多态性及其与乳腺炎关联性分析[J]. 遗传, 2009, 31(4): 393-399.
[2]	李艳华，张胜利，韩广文，薛建华，赵鹏. 利用单链构象多态性检测牛白细胞黏附缺陷症方法的建立[J]. 遗传, 2007, 29(12): 1471-1474.
[3]	王兴平，许尚忠，马腾壑，高雪，任红艳，陈金宝. 牛TLR4基因5′侧翼区的遗传变异与乳房炎的关联 [J]. 遗传, 2006, 28(12): 1520-1520~1524.
[4]	宋昉，金煜炜，王红，张玉敏，杨艳玲，张霆. 苯丙氨酸羟化酶（PAH）基因外显子7及其两侧内含子的突变研究[J]. 遗传, 2005, 27(1): 53-56.
[5]	江高峰，庄志雄，刘起展，徐雷，何云，杜柳涛. 中国广东汉族人群核苷酸修复基因hMTH1 遗传多态性研究[J]. 遗传, 2005, 27(1): 25-29.
[6]	孔祥东，杨宇霞，张思仲. AGT基因单倍型与原发性高血压[J]. 遗传, 2004, 26(6): 797-802.
[7]	赵迎社，杨中汉，冯建生，蒋建伟，吴美玉，周天鸿. 高脂血症患者脂蛋白脂酶基因外显子4区域变异的研究 [J]. 遗传, 2002, 24(5): 519-522.
[8]	王少丽，乔传令，盛承发. 单链构象多态性（SSCP）及其在昆虫学中的应用[J]. 遗传, 2002, 24(4): 483-485.
[9]	赵凯，李军祥，张亚平，罗静，李太平，吴华，田海宁. 青海湖裸鲤mtDNA遗传多样性的初步研究[J]. 遗传, 2001, 23(5): 445-633.
[10]	杨平，Martin Armstrong，戴德哉，Walter Luyten. 利用PCR-SSCP对人MINK 基因的研究[J]. 遗传, 1999, 21(5): 14-488.
[11]	周天鸿，李月琴，孙宏，朱志惠. PCR-RFLP检测LDL受体基因TaqⅠ多态性位点的研究[J]. 遗传, 1998, 20(2): 9-12.
[12]	鲁晓宣，茅一萍，张军，李明发，高翼之，王世浚. 中国人α珠蛋白基因3’HVR多态分布的研究[J]. 遗传, 1995, 17(3): 13-15.

荷斯坦牛HSP70-1基因遗传多态性与乳腺炎抗性关系

Research on Polymorphism of HSP70-1 gene in Holstein and the Relationship between Different Genotypes and Resistance to Dairy Mastitis

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 12

编辑推荐

Metrics