遗传 ›› 2009, Vol. 31 ›› Issue (8): 844-848.doi: 10.3724/SP.J.1005.2009.00844

• 研究报告 • 上一篇    下一篇

小麦糯性基因的多重PCR分子鉴定

卢龙斗1; 侯彩玲1;陈龙2;殷贵鸿3;邓传良1;高武军1;杨绪勤1;谭光轩2   

  1. 1. 河南师范大学生命科学学院, 新乡453007;
    2. 河南省周口师范学院生命科学系, 周口466000;
    3. 河南省周口市农业科学院, 周口466001
  • 收稿日期:2008-12-23 修回日期:2009-04-05 出版日期:2009-08-10 发布日期:2009-08-10
  • 通讯作者: 卢龙斗

Molecular identification on Waxy genes in wheat using multiple-PCR

LU Long-Dou1;HOU Cai-Ling1;CHEN Long2;YIN Gui-Hong3;DENG Chuan-Liang1;GAO Wu-Jun1;YANG Xu-Qin1;TAN Guang-Xuan2   

  1. 1. College of Life Science, Henan Normal University, Xinxiang 453007, China;
    2. Department of Life Science, Zhoukou Normal University, Henan Province Zhoukou 466000, China;
    3. Zhoukou Institute of Agricultural Science, Henan Province Zhoukou 466001, China
  • Received:2008-12-23 Revised:2009-04-05 Online:2009-08-10 Published:2009-08-10
  • Contact: LU Long-Dou1

摘要: 采用多重 PCR 的方法, 对其反应条件进行优化, 以获得用于小麦糯性(Wx)基因分析的稳定PCR体系。应用两对引物, 分别扩增小麦 Wx-A1、Wx-B1、Wx-D1 基因, 目的片段大小分别为: 230 bp/265 bp、854 bp和 204 bp。经反复验证, 结果准确可靠, 重复性好, 成本低, 可以在同一PCR反应体系中对 3 个Wx 基因进行同时筛选鉴定。该体系可用于 Wx 蛋白基因的分子标记辅助选择, 可以提高小麦淀粉品质评价和糯麦选育的效率。

关键词: 小麦, Wx基因, 多重PCR, 分子标记辅助选择

Abstract: Multiple-PCR was conducted to establish a stable PCR system for identifying the three Wx genes in wheat. Two pairs of primers were employed to amplify Wx-A1, Wx-B1, and Wx-D1 genes of wheat, with the target sequences of 230 bp/265 bp, 854 bp, and 204 bp, respectively. The results showed that Wx-A1, Wx-B1, and Wx-D1 can be detected si-multaneously in a single reaction. This method proved to be repeatable and low cost for evaluation of wheat quality proper-ties in breeding program. This multiple-PCR technique can be efficiently used in marker-assisted selection for Wx genes, which will improve selection procedure for waxy wheat.