图位克隆技术在农作物基因分离中的应用与评价

doi:10.3724/SP.J.1005.2010.00903

遗传 ›› 2010, Vol. 32 ›› Issue (9): 903-913.doi: 10.3724/SP.J.1005.2010.00903

图位克隆技术在农作物基因分离中的应用与评价

何俊平^{1, 2}, 阮松林², 祝水金¹, 马华升²

1. 浙江大学农业与生物技术学院, 杭州 310029; 2. 杭州市农业科学研究院生物技术研究所植物分子生物学与蛋白质组学实验室, 杭州 310024

收稿日期:2009-12-22 修回日期:2010-02-23 出版日期:2010-09-20 发布日期:2010-09-20
通讯作者: 祝水金;马华升 E-mail:shjzhu@zju.edu.cn;hzhsma@163.com
基金资助:
第44批中国博士后科学基金资助项目(编号：20080441230)和国家转基因重点大专项重点项目(编号：2009ZXD8001-027B)资助

The application and evaluation of map-based gene isolation in crops

HE Jun-Ping^{1, 2}, RUAN Song-Lin², ZHU Shui-Jin¹, MA Hua-Sheng²

1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; 2. Plant Molecular Biology and Proteomics, Institute of Biotechnology, Hangzhou Academy of Agricultural Science, Hangzhou 310024, China

Received:2009-12-22 Revised:2010-02-23 Online:2010-09-20 Published:2010-09-20
Contact: ZHU Shui-Jin,MA Hua-Sheng E-mail:shjzhu@zju.edu.cn;hzhsma@163.com

摘要/Abstract

摘要： 图位克隆(Map-based cloning)作为分离基因的有效方法, 已在小基因组作物的基因分离中得到了广泛应用和发展, 而在具有大量重复DNA序列的大基因组作物中的应用仍存在挑战。基于图位克隆在基因分离中的重要性, 文章就图位克隆技术的基本内容及发展做简要概述, 着重对图位克隆技术在大基因组作物中的应用进行分析和评价, 同时对它今后可能的发展方向进行了讨论, 以期为大基因组作物基因的分离提供借鉴。

关键词: 图位克隆, 基因分离, 大基因组

Abstract: As an efficient strategy for gene isolation, map-based cloning has been widely applied and well advanced in crops with small genomes. However, the application of this strategy is challenging in crops with large genomes that are rich in repetitive DNA. Because of the importance of map-based cloning, its basic contents and development are summarized. In particular, the application of this strategy is analyzed and evaluated in large genome crops, and the potential directions of its development are discussed. These help to promote genes cloning in large genome crops.

Key words: map-based cloning, gene isolation, large genome

何俊平，阮松林，祝水金，马华升. 图位克隆技术在农作物基因分离中的应用与评价[J]. 遗传, 2010, 32(9): 903-913.

HE Dun-Beng, RUAN Song-Lin, CHU Shui-Jin, MA Hua-Sheng. The application and evaluation of map-based gene isolation in crops[J]. HEREDITAS, 2010, 32(9): 903-913.

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图位克隆技术在农作物基因分离中的应用与评价

The application and evaluation of map-based gene isolation in crops

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