大肠杆菌F18菌株敏感和抵抗基因型仔猪十二指肠基因表达谱差异分析

doi:10.3724/SP.J.1005.2011.00060

遗传 ›› 2011, Vol. 33 ›› Issue (1): 60-66.doi: 10.3724/SP.J.1005.2011.00060

大肠杆菌F18菌株敏感和抵抗基因型仔猪十二指肠基因表达谱差异分析

包文斌^1,3,4, 叶兰¹, 潘章源¹, 朱璟¹, 杜子栋², 蔡家嘉¹, 黄小国^3,4, 朱国强^2,3,4, 吴圣龙^1,3,4

1. 扬州大学动物科学与技术学院, 扬州 225009;
2. 扬州大学兽医学院, 扬州 225009;
3. 江苏省现代猪种分子选育工程技术研究中心, 常州 213149;
4. 江苏(扬州)规模猪场高效健康养殖公共技术服务中心, 扬州 225009

收稿日期:2010-06-11 修回日期:2010-09-14 出版日期:2011-01-20 发布日期:2010-01-20
通讯作者: 吴圣龙 E-mail:slwu@yzu.edu.cn
基金资助:
国家自然科学基金项目(编号: 30972089), 转基因生物新品种培育科技重大专项(编号: 2009ZX08006-004B)和江苏省科技支撑计划(农业)项目(编号: BE2008364, BE2009330-2)资助

cDNA microarray on differently expressed genes in duodenum in porcine sensitive or resistant to Escherichia coli F18

BAO Wen-Bin^1,3,4, YE Lan¹, PAN Zhang-Yuan¹, ZHU Jing¹, DU Zi-Dong², CAI Jia-Jia¹, HUANG Xiao-Guo^3,4, ZHU Guo-Qiang^2,3,4, WU Sheng-Long^1,3,4

1. Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China;
2. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
3. Jiangsu Engineering Research Centre for Molecular Breeding of Breeder Pig, Changzhou 213149, China;
4. Jiangsu (Yangzhou) Public Technical Service Centre for Efficient and Healthy Culture Technology in Large-scale Swine Farms, Yang-zhou 225009, China

Received:2010-06-11 Revised:2010-09-14 Online:2011-01-20 Published:2010-01-20
Contact: WU Sheng-Long E-mail:slwu@yzu.edu.cn

摘要/Abstract

摘要： 文章运用Agilent 双标记表达谱芯片, 基于已建立的苏太猪大肠杆菌F18菌株敏感性和抗性型全同胞配对个体, 分析十二指肠组织基因表达谱差异, 旨在筛选导致仔猪断奶后腹泻和水肿病发生的大肠杆菌F18菌株受体相关基因, 探讨造成大肠杆菌病抗性和敏感性资源家系抗性差异的分子生物学机理。研究结果显示, 以Fold change绝对值大于2倍进行筛选, 在敏感型(GG基因型)对抗性型(AA基因型)配对组中, 差异基因共13个, 其中上调6个, 下调7个, 在以敏感型(AG基因型)对抗性型(AA基因型)配对组中, 共筛选出差异基因6个, 其中上调4个, 下调2个。经GO分析发现差异基因的生物学过程主要涉及免疫应答、胞外区修饰(如糖基化)、细胞黏附、信号转导等。通路发现大肠杆菌F18菌株抵抗性和敏感性差异基因主要涉及糖脂合成代谢以及炎症免疫相关通路, 经芯片筛选出的相关基因的功能还需进一步的研究验证。

关键词: 猪, 大肠杆菌F18菌株, 基因芯片, 基因表达谱

Abstract: Based on the paired full-sib individuals selected from the established resource populations of Sutai pig that were characterized as resistant or sensitive to ETEC F18, Agilent double labeled cDNA microarray was used to identify the gene expression profiles in duodenum on purpose of investigating the genes related to Escherichia coli F18 receptor, which may cause edema disease and post-weaning diarrhea in piglets, as well as exploring the molecular mechanism about the differences involved in two different lineages. The results showed that thirteen differently expressed genes were found in one matched group including sensitive ones with GG genotype comparing with resistant ones with AA genotype at a two-fold filter, where there were 6 up-regulated genes and 7 down-regulated genes. In the other matched group composed of sensitive ones with AG genotype, 4 up-regulated genes and 2 down-regulated genes, 6 in total were screened out. GO analy-sis revealed that the differently expressed genes participated in many biological processes, such as immune response, ex-tracellular region, bacterial binding, response to external stimulus and so on. Meanwhile, these genes were mainly related to the Glycan Biosynthesis and Metabolism and Immune System pathways. Actually, the roles that they may play in edema disease and post-weaning diarrhea need further study and verification.

Key words: porcine, Escherichia coli F18, gene chip, gene expression profile

包文斌，叶兰，潘章源，朱璟，杜子栋，蔡家嘉，黄小国，朱国强，吴圣龙. 大肠杆菌F18菌株敏感和抵抗基因型仔猪十二指肠基因表达谱差异分析[J]. 遗传, 2011, 33(1): 60-66.

BAO Wen-Bin, XIE Lan, BO Zhang-Yuan, SHU Jing, DU Zi-Dong, CA Jia-Jia, HUANG Xiao-Guo, SHU Guo-Jiang, TUN Ku-Long. cDNA microarray on differently expressed genes in duodenum in porcine sensitive or resistant to Escherichia coli F18[J]. HEREDITAS, 2011, 33(1): 60-66.

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大肠杆菌F18菌株敏感和抵抗基因型仔猪十二指肠基因表达谱差异分析

cDNA microarray on differently expressed genes in duodenum in porcine sensitive or resistant to Escherichia coli F18

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