双链DNA微阵列：原理、技术和应用

doi:10.3724/SP.J.1005.2013.00287

遗传 ›› 2013, Vol. 35 ›› Issue (3): 287-306.doi: 10.3724/SP.J.1005.2013.00287

双链DNA微阵列：原理、技术和应用

潘艳, 王进科

东南大学生物电子学国家重点实验室, 南京 210096

收稿日期:2012-09-05 修回日期:2012-12-11 出版日期:2013-03-20 发布日期:2013-03-25
通讯作者: 王进科 E-mail:wangjinke@seu.edu.cn
基金资助:
国家自然科学基金项目(编号：61171030)资助

Double-stranded DNA microarray: principal, techniques and applications

PAN Yan, WANG Jin-Ke

The State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China

Received:2012-09-05 Revised:2012-12-11 Online:2013-03-20 Published:2013-03-25

摘要/Abstract

摘要： 双链DNA微阵列也称为蛋白结合微阵列, 是高通量检测分析DNA结合蛋白(如转录因子)与大量DNA分子相互作用的一种重要技术。它将大量双链DNA分子固定在特定的固相支持物(如玻片)上, 与待测蛋白相互作用, 用以确定转录因子的DNA结合亲和性、特异性及序列偏好性。近年来, 该技术在快速表征大量转录因子的DNA特异性、绘制转录因子DNA结合谱、鉴定转录因子DNA结合位点和靶基因、识别转录因子家族内不同成员及其二聚体的细微DNA结合差异、考察辅助因子对转录因子DNA结合特异性影响等方面展现了其重要的应用价值。文章对双链DNA微阵列的原理、技术及应用进行了综述。

关键词: 双链DNA微阵列, 转录因子

Abstract: Double-stranded DNA (dsDNA) microarray, also known as protein binding microarray (PBM), is an important technique that can be used to assay the interaction of DNA-binding protein (such as transcription factor, TF) with vast amount of DNA molecules in high-throughput format. This technique immobilizes large amount of various dsDNA molecules on the surface of a solid support (such as glass slide) for detecting the binding interaction of a DNA-binding protein with all of the immobilized dsDNA molecules, and thus determining the DNA-binding affinity, specificity and preference of TFs. In recent years, this technique has demonstrated its valuable applications in several aspects, including rapidly characterizing DNA-binding specificity of large number of TFs, building DNA-binding profiles of TFs, identifying DNA-binding sites and target genes of TFs, discriminating the subtle DNA-binding preferences of members and their dimmers of a TF family, and examining the effects of a cofactor on the DNA-binding specificity of TFs. This paper reviews the principal, techniques, and applications of dsDNA microarray.

Key words: dsDNA microarray, transcription factor

潘艳，王进科. 双链DNA微阵列：原理、技术和应用[J]. 遗传, 2013, 35(3): 287-306.

PAN Yan, WANG Jin-Ke. Double-stranded DNA microarray: principal, techniques and applications[J]. HEREDITAS, 2013, 35(3): 287-306.

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双链DNA微阵列：原理、技术和应用

Double-stranded DNA microarray: principal, techniques and applications

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