摘要： 在NBL中建立初始悬浮培养物,再转移到AA2中建立原生质体分离用的胚性细胞悬浮系,利用这个方法成功地建立了Java14、毫梅、D.V.85、0242 8等的胚性细胞悬浮系。从Java14中分离的原生质体在KPR培养基中获得大量再生细胞团,并成功地实现了植株再生。通过渗透压的调整和培养过程中的加液处理,获得了0.8%较高的植板率。
Abstract:Embryogenic cell suspensions of Java 14,Haomei,D.V.85,02428 etc.were established via suspension methods of first establishing primary suspension cultures on NBL and then establishing embryogenic cell suspension for protoplast isolation on AA2 medium.A large number of clones were obtained when protoplasts of Java14 were cultured on KPR medium and plantiets were regenerated from clones.Plating efficiency was up to 0.8% by adjusting osmolality and lowing osmolality in protoplast culture.
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