遗传 ›› 1997, Vol. 19 ›› Issue (4): 4-4.

• 论文 • 上一篇    下一篇

小鼠胚胎核移植实验的初步报告

陆长富; 卢光琇; 卢惠霖 LU Chang-Fu;LU Guang-Xiu;LU Hui-Lin   

  1. 湖南医科大学人类生殖工程研究室, 长沙 410078 The Human Reprodnctive Engineering Laboratory,Hunan Medical University,Changsha 410078
  • 收稿日期:1900-01-01 出版日期:1997-08-10 发布日期:1997-08-10

Report on the Embryonic Nuclear Transplantation in Mouse

  • Received:1900-01-01 Online:1997-08-10 Published:1997-08-10

摘要: Abstract:Oocytes recovered from Fallopian tubes of female mice of Kun-Ming-Bai strain were enucleated by microsurgery and a single blastomere aspirated from 2-cell stage embryo derived from C57 was injected into the perivitelline space of earh enucleated oocyte.Then those coupled cells were exposed to PEG solution and cultured in an atmosphere of 5% Co2 at 37℃ for four hours.After that,couples which had been fused to one cell were transferred to the oviducts of pseudopregnant foster mothers.Six offsprings were obtained from this transplantation.
Key words:Mouse,Embryo,Nuclear transplantation

关键词: 分子进化, 28SrRNA二级结构, 蓖麻蚕rRNA基因, DNA序列

Key words: 28S rRNA secondary structure, Ribosomal gene of Attacus ricini, Molecular evolution, DNA sequence