遗传 ›› 1998, Vol. 20 ›› Issue (2): 1-4.

• 论文 •    下一篇

三系杂交稻亲本随机扩增多态性DNA(RAPD)分析

马文宾1;2; 庄杰云1; 彭应财1; 王侯聪2; 郑康乐1 MA Wen-bin1;2;ZHUANG Jie-yun1;PENG Ying-cai1;WANG Hou-cong2;ZHENG Kang-le1   

  1. 1.中国水稻研究所, 杭州 310006 2.厦门大学生物系,厦门 361005 1.Biotechnology Department,China National Rice Research Institute,Hangzhou 310006 2.Biology Department,Xiamen University,Xiamen 361005
  • 收稿日期:1900-01-01 出版日期:1998-04-10 发布日期:1998-04-10

RAPD Studies of Parents of Three-Line Hybrid Rice

  • Received:1900-01-01 Online:1998-04-10 Published:1998-04-10

摘要: 选用9个随机引物对31份杂交水稻亲本材料进行了RAPD分析, 共检测到60条多态性带。聚类分析结果表明,所有供试材料可以被明确地区分。在9个随机引物中,有8个具有较高的多态性检测能力。以这8个引物为基础,选用任两个引物即可在任一对材料中检测出多态性的频率在96.13%以上,而选用任3个引物则该频率在99.21%以上。这显示了运用RAPD鉴定稻种具有简便、灵敏、高效的优点,在鉴定杂交稻种的实践中有着良好的应用前景。
Abstract:Seven rice sterile lines,12 maintainer lines and 12 restorer lines were analyzed by RAPD with 9 primers.Altogether,118 fragments were generated,of which 60 detected polymorphisms among rice marker.Eight of nine primers can detect high polymorphism.The frequencies of polymorphism in any primers were used,the frequencies would be higher than 99.21%.The eight primers were therefore recommended as candidates for the identification of hybrid rice seeds.

关键词: 杂交水稻, RAPD, Random amplified polymorphic DNA(RAPD), Rice, 多态性频率
Key words,
PCR-SSP, HLA-Cw, 血清学鉴定抗原, 非血清学鉴定抗原

Key words: HLA-Cw PCR-SSP Serologically defined antigen Non-serologically defined antigen