一步PCR快速扩增辽宁碱蓬甜菜碱醛脱氢酶cDNA 3'末端序列

遗传 ›› 2002, Vol. 24 ›› Issue (2): 179-181.

一步PCR快速扩增辽宁碱蓬甜菜碱醛脱氢酶cDNA 3'末端序列

李秋莉1;2;高晓蓉2;袁晓东3;刘大伟2;安利佳2 LI Qiu-li1;2;GAO Xiao-rong2;YUAN Xiao-dong3;LIU Da-wei2;AN Li-jia2

1.辽宁师范大学生物系，大连 116029； 2.大连理工大学生物工程系，大连 116012; 3.宝生物工程大连有限公司，大连 116600 1.Biology Department of Liaoning Normal University,Dalian 116029,China; 2.Bioengineering Department of Dalian University of Technology,Dalian 116012,China; 3.TaKaRa Biotechnology Dalian Co.,Ltd.,Dalian 116600,China

收稿日期:1900-01-01 出版日期:2002-04-10 发布日期:2002-04-10

Rapid Amplification of 3′cDNA End of Suaeda liaotungensis Betaine Aldehyde Dehydrogenase Using One-step PCR

Received:1900-01-01 Online:2002-04-10 Published:2002-04-10

摘要/Abstract

摘要： 根据已获得的辽宁碱蓬甜菜碱醛脱氢酶cDNA的部分序列，设计一条基因特异性引物,与通用引物并用，一步PCR成功地克隆了辽宁碱蓬甜菜碱醛脱氢酶cDNA 3′末端。与常规的3′RACE法相比，一步PCR法具有快速、简便、经济等优点，是一种非常快捷的扩增cDNA 3′末端序列的方法。
Abstract:Based on part of a known cDNA sequence of Suaeda liaotungensis betaine aldehyde dehydrogenase,we successfully cloned the 3′cDNA end of S.lianotungensis betaine aldehyde dehydrogenase using onestep PCR with a genespecific primer and universal primer.Compared with the typical 3′ RACE,onestep PCR is rapid,simple and inexpensive.It is very rapid to amplify an unknown cDNA 3′end using this method.

关键词: 甜菜碱醛脱氢酶, onestep PCR, 3′cDNA 末端
Key words, 一步PCR, 辽宁碱蓬

李秋莉，高晓蓉，袁晓东，刘大伟，安利佳. 一步PCR快速扩增辽宁碱蓬甜菜碱醛脱氢酶cDNA 3'末端序列[J]. 遗传, 2002, 24(2): 179-181.

LI Qiu-li, GAO Xiao-rong, YUAN Xiao-dong, LIU Da-wei, AN Li-jia. Rapid Amplification of 3′cDNA End of Suaeda liaotungensis Betaine Aldehyde Dehydrogenase Using One-step PCR[J]. HEREDITAS, 2002, 24(2): 179-181.