摘要: 本研究对猪LHβ亚基基因除上游调控区以外的序列进行了SSCP分析,结果发现在该基因的3个外显子和3′调控区内没有任何SNPs位点,仅在内含子1的1367位点和内含子2的1823位点发现2个多态性位点,并对这两个位点在二花脸、约克夏和长白猪3个群体中的基因频率进行了初步分析。
Study on the Single Nucleotide Polymorphisms of the Porcine LHβ Gene
WANG Ai-hua1,LI Ning2,WU Chang-xin1
1.College of Animal Science and Technology,China Agricultural University,Beijing 100094;
2.National Laboratory of AgroBiotechnology,China Agricultural University,Beijing 100094
Abstract:SSCP analysis was commanded on the whole sequence of porcine LHβ gene except the upstream control region.No polymorphic site was found in the 3 exons and 3′control region.But there were 2 polymorphic sites in the 1367 site of intron 1 and 1823 site of intron 2.The gene frequencies of the 2 sites in the Erhualian,Yorkshire and Landrace populations were analyzed.
Key words:LHβ gene;SNPs;intron;SSCP