摘要： 介绍了一种全新、简单的提取水稻种胚RNA的CTAB-LiCl提取法，可在不用液氮的室温下有效地排除剥取的种胚中大量带有的多糖和本身脂质的干扰，所得RNA样品经紫外分光光度计和琼脂糖凝胶电泳分析证明具有较高的纯度和完整性，并可进一步满足RT-PCR和Northern 印迹的实验需要，适用于富含多糖和脂质的植物组织RNA的提取。
Abstract:A novel and simple CTAB-LiCl-based extraction method for high-quality and total RNA of rice embryo samples is developed.This method can efficiently eliminate the interference of polysaccharide and lipids rich in rice（Oryza sativa L.）embryo obtained under room temperature without using liquid nitrogen.The results of ultraviolet spectrophotometer and agarose gel electrophoresis analysis show that the obtained RNA has no obvious degradation and a good purity sufficient for further RT-PCR and RNA gel blotting.Therefore,it is also especially useful for the RNA extraction of plant material plenty of polysaccharide and lipids.