遗传 ›› 2004, Vol. 26 ›› Issue (4): 529-531.

• 论文 • 上一篇    下一篇

改良CTAB法用于多年生植物组织基因组DNA的大量提取*

陈昆松1 李 方1 徐昌杰1 张上隆1 傅承新2 CHEN Kun-Song1; LI Fang1; XU Chang-Jie1; ZHANG Shang-Long1; FU Cheng-Xin2   

  1. 1. 浙江大学果实生理与生物技术实验室/农业部园艺植物生长发育与生物技术重点开放实验室,华家池校区, 杭州 310029; 2. 浙江大学生命科学学院,华家池校区, 杭州 310029 1 Laboratory of Fruit Molecular Physiology and Biotechnology/ The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Huajiachi Campus, Hangzhou 310029,China; 2 College of Life Sciences, Zhejiang University, Huajiachi Campus, Hangzhou 310029,China
  • 收稿日期:1900-01-01 出版日期:2004-08-10 发布日期:2004-08-10

An Efficient Macro-method of Genomic DNA Isolation from Actinidia chinensis Leaves

  • Received:1900-01-01 Online:2004-08-10 Published:2004-08-10

摘要:

根据多年生植物组织富含多酚、多糖的具体特性,对现有的DNA提取方法进行了改进。通过增加提取缓冲液中b-巯基乙醇用量,简化氯仿/异戊醇抽提液步骤,改用经-20℃预冷异丙醇沉淀DNA等,对CTAB法加以改进。改进后方法具有以下优点:(1)获得的DNA质量良好,提取过程无明显的DNA降解,基本上排除了多酚物质的干扰;(2)用获得的DNA进行Southern杂交,可得到理想的杂交信号,可满足相关的分子研究要求;(3)操作简便。Abstract It is a difficult problem to isolate high quality DNA from plants containing a high contents of polyphenolics and polysaccharose, such as Actinidia plant. The protocol described in this paper is a modified CTAB (hexadecyltrimethylammonium bromide) method. High quality genomic DNA can be isolated from Actinidia plant using the improved method. The DNA is good enough for Southern blot and other uses in DNA research. The protocol is also efficient for quick and macro-DNA extraction.

关键词: DNA, 猕猴桃, Southern杂交
Key words,
Actinidia, 提取