蒋华云1;曹祥荣1;张锡然1;胡 均2 ;徐春茂2 JIANG Hua-Yun1;CAO Xiang-Rong1;ZHANG Xi-Ran1; HU Jun2; XU Chun-Mao2
Abstract: According to the human sex differentiation related ZFY and ZFX genes, a pair of primers were designed , and fragments were amplified from the genomic DNA of male or female tufted deer. Subsequently the amplified fragments were cloned into the vector pMD18T and were sequenced. It is found that the sequences of ZFY gene and ZFX gene have 91% homology. Based on the different nucleotides, restriction site of AvaⅡ was found to be specific to ZFX gene. The results show that the combination of PCR with AvaⅡ digestion is a simple and sensitive way to identify the tufted deer sex.
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