PlcR在炭疽芽胞杆菌A16R中的功能研究

doi:10.16288/j.yczz.15-040

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HEREDITAS(Beijing) ›› 2015, Vol. 37 ›› Issue (5): 494-498.doi: 10.16288/j.yczz.15-040

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The function of PlcR in Bacillus anthracis vaccine strain A16R

Xiaolin Jia^{1, 2}, Dongshu Wang², Zhiqi Gao², Erling Feng², Jiping Zheng^{1, 3}, Hengliang Wang², Guiying Guo¹, Xiankai Liu²

1. Ministry of Education Key Laboratory for Tropical Biological Resources, Hainan University, Haikou 570228,China;
2. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China;
3. Hainan Key Laboratory for Sustainable Utilization of Tropical Bio-resources-State Key Laboratory Incubation Base, Haikou 570228, China

Received:2015-01-19 Online:2015-05-20 Published:2015-05-20

Abstract

Abstract: Bacillus anthracis, B. thuringiensis and B. cereus are members of the B. cereus group. They share high genetic similarity. Whereas plcR (Phospholipase C regulator) usually encodes a functional pleiotropic activator protein in B. cereus and B. thuringiensis isolates, a characteristic nonsense mutation is found in all B. anthracis strains investigated, making the gene dysfunctional. To study the function of PlcR in B. anthracis, we used the B. cereus CMCC63301 genome as a template and constructed a recombinant expression plasmid pBE2A-plcR, and introduced it into the B. anthracis vaccine strain A16R, and then analyzed the activity of the hemolysin and sphingomyelinase. The results showed that transformation of B. anthracis with plasmid pBE2A-plcR carrying the native B. cereus plcR gene active the expression of sphingomyelinase gene, but did not activate expression of hemolysin genes of B. anthracis A16R.

Key words: Bacillus cereus, plcR, Bacillus anthracis, sphingomyelinase, hemolysin

Xiaolin Jia, Dongshu Wang, Zhiqi Gao, Erling Feng, Jiping Zheng, Hengliang Wang, Guiying Guo, Xiankai Liu. The function of PlcR in Bacillus anthracis vaccine strain A16R[J]. HEREDITAS(Beijing), 2015, 37(5): 494-498.

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The function of PlcR in Bacillus anthracis vaccine strain A16R

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