YAP蛋白T425A位点突变对YAP功能的影响

doi:10.16288/j.yczz.17-068

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Hereditas(Beijing) ›› 2017, Vol. 39 ›› Issue (7): 675-682.doi: 10.16288/j.yczz.17-068

The roles of YAP T425A mutation in the regulation of YAP activity

lu Yang¹(),Zhuangzhi Zou¹, ²(),Beibei Mao³(),

1. College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
2. Department of Oncology, Chinese PLA General Hospital, Beijing 100853, China
3. State key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Received:2017-02-28 Revised:2017-04-18 Online:2017-07-20 Published:2017-07-04
Supported by:
the National Natural Science Foundation of China(81402319);Beijing Novel Project(Z161100004916133)

Abstract

Abstract:

The Hippo signaling pathway plays a critical role in body development and tissue growth. As the core effector of the Hippo signaling pathway, Yes-associated protein (YAP) has been reported to be involved in various kinds of human cancers. However, the mechanism for the regulation of YAP activity has not been completely understood. In this study, we constructed a YAP Thr425Ala mutant and found that this mutation decreased YAP transcriptional activity. Further, T425A retained YAP in the cytoplasm without affecting the phosphorylation of YAP S127. Moreover, we observed that the T425A mutation attenuated the ability of YAP in driving MCF10A cell migration. Our research indicates that T425 of YAP is important for the regulation of YAP localization and activity.

Key words: YAP, YAP T425A, YAP transcriptional activity, YAP nuclear localization, cell migration

lu Yang, Zhuangzhi Zou, , Beibei Mao, . The roles of YAP T425A mutation in the regulation of YAP activity[J]. Hereditas(Beijing), 2017, 39(7): 675-682.

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