利用转录组测序筛选鸡蛋褐壳性状相关基因

doi:10.16288/j.yczz.17-111

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Hereditas(Beijing) ›› 2017, Vol. 39 ›› Issue (11): 1102-1111.doi: 10.16288/j.yczz.17-111

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Transcriptome sequencing identifies potential regulatory genes involved in chicken eggshell brownness

Guangqi Li^1,²(),Congjiao Sun¹(),Guiqin Wu²,Fengying Shi²,Aiqiao Liu²,Hao Sun²,Ning Yang¹()

1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
2. Beijing Huadu Yukou Poultry Industry Co. Ltd., Beijing 101206, China

Received:2017-03-28 Revised:2017-09-02 Online:2017-11-20 Published:2017-12-20
Supported by:
the National Natural Science Foundation of China(31501915);Chinese Universities Scientific Fund(2017QC009, 2017DK001);National Agricultural Technology System (Layer)(CARS-41);Programs for Changjiang Scholars and Innovative Research in University(IRT119)

Abstract

Abstract:

Brown eggs are popular in many countries, and consumers regard eggshell brownness as an important indicator of egg quality. Brown eggshell color is controlled by polygene. However, the responsible genes and detailed molecular mechanisms regulating eggshell brownness have not been defined. In the present study, we applied the RNA-seq technology to analyze the transcriptome data of the shell gland epithelium of hens and investigated the candidate genes associated with eggshell brownness. The results indicated that 8461 genes were expressed in the shell gland epithelium, of which 34 genes were differentially expressed in hens laying dark vs. light brown eggs. Functional analysis revealed that two genes, ovotransferrin (TF) and heat-shock protein 70 (HSP70), as well as the oxidative phosphorylation pathway were involved in the synthesis and transport of protoporphyrin Ⅸ, which might influence the formation of eggshell brownness and result in different shades of brown.

Key words: chicken eggs, eggshell brownness, transcriptome

Guangqi Li, Congjiao Sun, Guiqin Wu, Fengying Shi, Aiqiao Liu, Hao Sun, Ning Yang. Transcriptome sequencing identifies potential regulatory genes involved in chicken eggshell brownness[J]. Hereditas(Beijing), 2017, 39(11): 1102-1111.

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