精子变形过程中组蛋白-鱼精蛋白替换调控机制

doi:10.16288/j.yczz.21-205

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Hereditas(Beijing) ›› 2021, Vol. 43 ›› Issue (12): 1121-1131.doi: 10.16288/j.yczz.21-205

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Regulation of histone-to-protamine transition during spermiogenesis

Lu Yuan^1,²(), Tingting Ge^1,², Changmin Niu^1,², Wenhua Xu^1,², Ying Zheng^1,²()

1. Department of Histology and Embryology, School of Medicine, Yangzhou University, Yangzhou 225009, China
2. Jiangsu Key Laboratory of Experimental & Translational Non-coding RNA Research, Yanghzou 225009, China;

Received:2021-06-10 Revised:2021-10-27 Online:2021-12-20 Published:2021-11-10
Contact: Zheng Ying E-mail:2920054914@qq.com;yzzkl@163.com
Supported by:
Supported by the National Natural Science Foundation of China(82071696);Supported by the National Natural Science Foundation of China(82101674);the Key Scientific Project for Jiangsu Provincial Universities No(20KJA310002);the Postgraduate Scientific Research Innovation Program of Yangzhou University No(XKYCX20_35)

Abstract

Abstract:

Spermiogenesis is the final stage of spermatogenesis, in which round spermatids differentiate into mature sperms through a complex process including morphological changes and chromatin condensation. Histone-to-protamine transition during spermiogenesis is a critical part of this biological process. Histones are initially replaced by testis-specific histone variants, then transition proteins integrate into the nucleus, and are in turn replaced by protamine. Impaired histone-to-protamine transition may cause azoospermia, oligospermia or teratospermia, which lead to male infertility. In this review, we summarize the research progress of regulatory mechanisms of the histone-to-protamine transition, thereby providing the theoretical basis for the diagnosis and treatment of male infertility.

Key words: spermiogenesis, histone-to-protamine transition, post-translational modification

Lu Yuan, Tingting Ge, Changmin Niu, Wenhua Xu, Ying Zheng. Regulation of histone-to-protamine transition during spermiogenesis[J]. Hereditas(Beijing), 2021, 43(12): 1121-1131.

Figures/Tables 3

Table 1

Fig. 1

Fig. 2

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基因	基因敲除表型	功能	参考文献
H1t	雄性生育力正常	诱导局部染色质松弛	[2,3]
H1t2	雄性不育	参与PRM的掺入与染色质凝聚	[4,5]
Th2a/Th2b	雄性不育	调节染色质开放或总组蛋白水平	[8,9]
H2al2	雄性不育	组装开放的核小体并允许TP掺入	[10,11]
H2a.b	雄性生育力下降	调节H2AL2的解聚和TP1的掺入	[12,14]
H3f3a/H3f3b	雄性不育	有助于形成开放的染色质构型	[16,17]
H3t	雄性不育	-	[18,19]
Epc1	雄性不育	参与组蛋白的超乙酰化	[25]
Tip60	雄性不育	参与组蛋白的超乙酰化	[25]
Brdt	雄性不育	介导乙酰化组蛋白的替换	[33,34]
Rnf8	雄性不育	单泛素化H2A和H2B,调节组蛋白的解聚	[26,27]
Miwi	雄性不育	对RNF8的核转位必不可少,并促进组蛋白泛素化和解聚	[28]
L3mbtl2	雄性生育力下降	参与PRM1的沉积和染色质凝聚	[29]
Phf7	雄性不育	识别H3K4me2/me3并催化H2A泛素化以促进组蛋白的解聚	[37]
Pa200	雄性生育力下降	通过蛋白酶体介导乙酰化的组蛋白降解	[42,43]
Tnp1/Tnp2	雄性生育力下降	PRM2的掺入和染色质凝聚所必需	[46~49]
Dazl	雄性不育	调节TP mRNA的表达	[50]
Jmjd1a	雄性不育	调节精子基因组包装和染色质凝聚	[51,53]
Prm1/Prm2	雄性不育	染色质凝聚所必需	[55,56]
Camk4	雄性不育	介导PRM2磷酸化	[57]
Ppp1cc2	雄性不育	使鱼精蛋白2第56位丝氨酸(Prm2Ser56)去磷酸化	[58]
Ip6k1	雄性不育	调控TP2和PRM2的表达	[59]

Regulation of histone-to-protamine transition during spermiogenesis

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