LincRNA AC027700.1在小鼠蜕膜化中的表达研究

doi:10.16288/j.yczz.21-356

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Hereditas(Beijing) ›› 2022, Vol. 44 ›› Issue (2): 168-177.doi: 10.16288/j.yczz.21-356

• Research Article • Previous Articles Next Articles

The expression of lincRNA AC027700.1 in mouse decidualization

Liping Tan(), Rufei Gao, Xin Yin, Xuemei Chen, Fangfang Li, Liu Yuan, Junlin He()

Joint International Research Laboratory of Reproduction and Development, Chongqing Medical University, Chongqing 400016, China

Received:2021-10-15 Revised:2022-01-17 Online:2022-02-20 Published:2022-01-24
Contact: He Junlin E-mail:2957066450@qq.com;hejunlin@cqmu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China No(31571554);General Project of Chongqing Natural Science Foundation No(cstc2020jcy j-msxmX0041);Postgraduate Tutor Team Construction Project of Chongqing No(dstd201809)

Abstract

Abstract:

Long non-coding RNAs (lncRNAs), which belong to the non-protein-coding RNAs, are greater than 200 nt in length. Although they have been found to play crucial roles in the regulation of cell growth and development, cell metabolism and the development of diseases, they are rarely reported in decidualization. The objective of our study is to explore the expression of lincRNA AC027700.1 in the endometrium of early pregnant mice and its role in decidualization. The expression of AC027700.1 in uterine tissues at implantation sites and inter implantation sites on the 6th day of pregnancy were detected by qRT-PCR. The relative expression of AC027700.1 in an in vivo model of induced decidualization in pseudopregnant mice and in in vitro model of induced decidualization in primary stromal cells and nucleus/cytoplasmic fractions were detected by qRT-PCR. GO and KEGG analysis of downstream target genes were performed by GOseq and KOBAS, respectively. The results show that AC027700.1 expression is significantly increased in tissues at implantation sites on the 6th day of pregnancy and in decidualized endometrial tissues and stromal cells. Furthermore, AC027700.1 localizes in the nuclear fraction and the downstream targeted genes are mainly involved in autophagy, cell cycle and RNA transport pathways. This study revealed that lincRNA AC027700.1 may be involved in decidualization of endometrium in early pregnancy, but the specific role and regulatory mechanism remain to be further studied.

Key words: lincRNA, AC027700.1, decidualization, endometrium, implantation

Liping Tan, Rufei Gao, Xin Yin, Xuemei Chen, Fangfang Li, Liu Yuan, Junlin He. The expression of lincRNA AC027700.1 in mouse decidualization[J]. Hereditas(Beijing), 2022, 44(2): 168-177.

Figures/Tables 6

Table 1

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References 33

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基因	序列(5'→ 3')
β-actin	F: GTGCTATGTTGCTCTAGACTTCG
β-actin	R: ATGCCACAGGATTCCATACC
Dtprp	F: TACCCACGTAAGGTCATC
Dtprp	R: CTCAGAGCCAGAAATCA
AC027700.1	F: CGGAAGTTCAGCAGAAGGAAGAGG
AC027700.1	R: GCCCACACCTTATTCACCAAGACC
U3	F: CTGGCTCTAGGTGCTGCTTGATTG
U3	R: ACTGCGTTCCCTCCCTCTCAC
U4	F: GCGCAGTGGCAGTATCGTAGC
U4	R: CAAGTCGTCATGGCGGGGTATTG

The expression of lincRNA AC027700.1 in mouse decidualization

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