梅山猪胚胎附植期EphB2的组织表达及RNA-seq分析

doi:10.3724/SP.J.1005.2014.1243

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HEREDITAS(Beijing) ›› 2014, Vol. 36 ›› Issue (12): 1243-1248.doi: 10.3724/SP.J.1005.2014.1243

• Research Articles • Previous Articles Next Articles

Tissue expression of EphB2 and RNA-seq analysis during embryo im-plantation in Meishan pigs

Yanfeng Fu¹, Yanhong Zhou^{1, 2}, Aiguo Wang³, Lan Li^{4, 5}, Honglin Liu², Bixia Li¹, Shouwen Ren¹

1. The Key Laboratory of Animal Breed Improvement and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China3. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
4. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;
5. National Research Center of Veterinary Biologicals Engineering and Technology, Nanjing 210014, China

Received:2014-03-22 Online:2014-12-20 Published:2014-12-20

Abstract

Abstract: Embryo implantation is a key step affecting swine litter size, which is an important economic and reproduction trait in pigs. In order to investigate the effect of erythropoietin-producing hepatocellular receptor B2 (EphB2) on endometrium migration and attachment during swine embryo implantation, the mRNA and protein expression levels of EphB2 in endometrium implantation sites, endometrium non-implantation sites and ovary were detected in Meishan sows during pre-implantation, mid-implantation and post-implantation period using real-time quantitative PCR and Western blot. Differential expression genes were also analyzed in endometrium implantation sites and ovary during different implantation periods by RNA sequencing (RNA-seq) technology. The qRT-PCR and Western blot results showed that EphB2 mRNA and protein expression curve was the same in endomtrium implantation sites and endometrium non-implantation sites during pre-implantation, mid-implantation and post-implantation period, with a first increase followed by a decrease, and its expression level during mid-implantation was significantly higher than pre-implantation and post-implantation (P<0.01). In contrast, EphB2 mRNA and protein expression curve in ovary during pre-implantation, mid-implantation and post-implantation period showed a first decrease followed by an increase, and the expression levels were significantly different among different implantation periods (P<0.05). RNA-seq results indicated that EphB2 mRNA expression during mid-implantation was higher than that of pre-implantation extremely significantly in endometrium implantation sites (P<0.01), and was significantly higher than that of post-implantation in ovary (P<0.05). By and large, EphB2 might play an important role in swine embryo implantation, and it’s a potential candidate gene for litter size in pigs.

Key words: EphB2, embryo implantation, expression, RNA-seq, Meishan pig

Yanfeng Fu, Yanhong Zhou, Aiguo Wang, Lan Li, Honglin Liu, Bixia Li, Shouwen Ren. Tissue expression of EphB2 and RNA-seq analysis during embryo im-plantation in Meishan pigs[J]. HEREDITAS(Beijing), 2014, 36(12): 1243-1248.

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Tissue expression of EphB2 and RNA-seq analysis during embryo im-plantation in Meishan pigs

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