海岛棉几丁质酶基因GbCHI的克隆与功能分析

doi:10.3724/SP.J.1005.2012.00240

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HEREDITAS ›› 2012, Vol. 34 ›› Issue (2): 240-247.doi: 10.3724/SP.J.1005.2012.00240

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Cloning and functional analysis of chitinase gene GbCHI from sea-island cotton (Gossypium barbadense)

MA Yin-Ping^1,2, WANG Fu-Xin², YANG Chun-Lin², SHEN Fa-Fu¹, XIA Gui-Xian²

1. State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, China 2. State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

Received:2011-03-22 Revised:2011-05-23 Online:2012-02-20 Published:2012-02-25

Abstract

Abstract: Chitinase is one of the important pathogenesis-related (PR) proteins in plants. By comparative proteomics study, a novel pathogen-responsive chitinase (known as GbCHI) has been identified from sea-island cotton (Gossypium barbadense). The GbCHI cDNA was cloned from wilt-resistant sea-island cotton and the anti-fungal activity of the gene product was investigated. qRT-PCR analysis indicated that GbCHI was expressed constitutively in root, stem, leaf, flower, and ovule of cotton plant, and the expression could be induced by Verticillium dahliae and plant hormone SA, ACC, and JA. Subcellular localization analysis using GFP-tagged proteins showed that GbCHI-GFP fusion proteins were targeted mainly to the plasma membrane. Anti-fungal assay demonstrated that GbCHI could inhibit spore germination and hyphae growth of V. dahliae significantly. These results provide important information for under-standing the cellular function of GbCHI and for exploring the application potential of this gene in molecular breeding of wilt-tolerant cotton plants.

Key words: sea-island cotton, verticillium dahliae, chitinases, cloning, anti-fungus activity

MA Yin-Ping, WANG Fu-Xin, YANG Chun-Lin, SHEN Fa-Fu, XIA Gui-Xian. Cloning and functional analysis of chitinase gene GbCHI from sea-island cotton (Gossypium barbadense)[J]. HEREDITAS, 2012, 34(2): 240-247.

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Cloning and functional analysis of chitinase gene GbCHI from sea-island cotton (Gossypium barbadense)

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