绵羊ILK基因的克隆及其在毛囊生长期的表达

doi:10.3724/SP.J.1005.2012.00719

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HEREDITAS ›› 2012, Vol. 34 ›› Issue (6): 719-726.doi: 10.3724/SP.J.1005.2012.00719

Cloning and expression in follicle anagen of ILK gene in sheep

YANG Jian-Bo^1,2,3, GAN Shang-Quan^2,3, YANG Yong-Lin^2,3, ZHANG Hong-Lin⁴, SONG Tian-Zeng⁵, FENG Jing⁵, YANG Jing-Quan^2,3, GAO Lei^2,3, SHI Guo-Qing^1,2,3, SHEN Min^2,3

1. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China 2. Animal Husbandry and Veterinary Institute, Xinjiang Academy of Agricultural And Reclamation Science, Shihezi 832000, China 3. Key Lab of Sheep Breeding and Reproduction, Xinjiang Agroreclamation Academy of Sciences, Shihezi 832000, China 4. School of Biochemical and Environmental Enginerring, Nanjing Xiaozhuang College, Nanjing 211171, China 5. Insitute of Animal Husbandry and Veterinary, Tibet Academy of Agriculture and Animal Science, Lhasa 850009, China

Received:2012-01-14 Revised:2012-05-07 Online:2012-06-20 Published:2012-06-25

Abstract

Abstract: Integrin linked kinase (ILK) is a scaffold protein, which plays important roles in hair follicle development. The cDNA sequence of novel ILK gene in sheep was cloned by PCR method and analyzed by bioinformatics. Tissue expression profiling in eight tissues and temporal profiling at different wool follicle anagen stages in skin was analyzed. The results showed that the whole open reading frame (ORF) of ILK gene was 1 359 bp in length, which encoded 452 amino acids. Bioinformatic analysis indicated that the secondary structure of ILK gene was mainly made up of three ankyrin repeats and a kinase domain, and there were multiple phosphorylation and Protein Kinase C sites in this gene. The RT-PCR result confirmed that ILK mRNA was expressed in heart, liver, spleen, lung, skeletal muscle, skin, and small intestine, and the expression level was much higher in skin, spleen, and liver than others. The q-PCR analysis demon-strated that the expression level of ILK was significantly increased from March to May (early follicle anagen initia-tion) in both sheep breeds, Chinese Merino and Kazakh sheep, and there were certain differences from June to October be-tween the two breeds. The above results indicated that ILK gene may play key roles in regulating secondary follicle growth.

Key words: hair follicle, sheep, integrin-linked kinase, gene cloning

YANG Jian-Bo, GAN Shang-Quan, YANG Yong-Lin, ZHANG Hong-Lin, SONG Tian-Zeng, FENG Jing, YANG Jing-Quan, GAO Lei, SHI Guo-Qing, SHEN Min. Cloning and expression in follicle anagen of ILK gene in sheep[J]. HEREDITAS, 2012, 34(6): 719-726.

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Cloning and expression in follicle anagen of ILK gene in sheep

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