斜带石斑鱼(Epinephelus coioides)GnRHⅡ基因的克隆及启动子分析

doi:10.3724/SP.J.1005.2013.00896

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HEREDITAS ›› 2013, Vol. 35 ›› Issue (7): 896-902.doi: 10.3724/SP.J.1005.2013.00896

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Cloning and promoter analysis of gonadotrophin-releasing hormone Ⅱ in the orange-spotted grouper (Epinephelus coioides)

CHEN Ji, LUO Yu-Shan, ZHU Zuo-Yan, HU Wei

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

Received:2012-12-28 Revised:2013-03-21 Online:2013-07-20 Published:2013-07-25

Abstract

Abstract: Gonadotrophin-releasing hormone (GnRH) is a key regulator of reproduction in all vertebrates. We first cloned the cDNA and genomic DNA sequences coding for GnRHⅡ gene in the orange-spotted grouper (Epinephelis coioides), an economically important marine fish, and then cloned its promoter sequence. The region responsible for the cell-specific expression of GnRHⅡ was located between -2005 bp to -956 bp from the translation start site. GnRHⅡ promoter driven EGFP expression in transgenic zebrafish showed that GnRHⅡ-positive neurons were primarily located in the midbrain and in the eyes. Our results provide an improved understanding of the regulatory mechanism and function of GnRHⅡ of E. coioides.

Key words: orange-spotted grouper(Epinephelus coioides), gonadotrophin-releasing hormone (GnRH), promoter

CHEN Ji, LUO Yu-Shan, ZHU Zuo-Yan, HU Wei. Cloning and promoter analysis of gonadotrophin-releasing hormone Ⅱ in the orange-spotted grouper (Epinephelus coioides)[J]. HEREDITAS, 2013, 35(7): 896-902.

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Cloning and promoter analysis of gonadotrophin-releasing hormone Ⅱ in the orange-spotted grouper (Epinephelus coioides)

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