森林生态系中球孢白僵菌遗传多样性的ISSR分析

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HEREDITAS ›› 2006, Vol. 28 ›› Issue (8): 977-983.

• 研究报告 • Previous Articles Next Articles

Genetic Diversity of Beauveria bassiana (Bals.) Vuill. in Forest Ecosystem Assessed by Inter-simple Sequence Repeat (ISSR) Markers

LI Min ¹, WANG Si-Bao ^1,2, FAN Mei-Zhen ¹，LI Zeng-Zhi ¹, HUANG Yong-Ping ²

1. Anhui Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036,China;
2 Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032,China

Received:2005-12-08 Revised:2006-02-06 Online:2006-08-10 Published:2006-08-10
Contact: LI Min

Abstract

Abstract:

In the present paper, the genetic diversity of 48 Beauveria bassiana strains from different altitudes and at different seasons in Dabie Mountains of western Anhui was estimated using inter-simple sequence repeat (ISSR) markers. Twelve among 33 ISSR primers were chosen for their reproducibility and high polymorphism. Seven (2～11) markers per primer were scored, and a total of 84 fragments were amplified, in which 73 (81%) were polymorphic. Genetic diversity analysis revealed a relatively high level of intraspecific genetic diversity of B. bassiana in Dabie Mountains of western Anhui: the percentage of polymorphic loci (PPL) was 81%, Nei′s genetic diversity (He) was 0.3187 and Shannon′s genetic diversity index (I) was 0.4782. The genetic differentiation, GST was 0.1028, indicating that a low degree of genetic differentiation occurred in the B. bassiana among populations.

CLC Number:

Q933

LI Min, WANG Si-Bao, FAN Mei-Zhen, LI Zeng-Zhi, HUANG Yong-Ping. Genetic Diversity of Beauveria bassiana (Bals.) Vuill. in Forest Ecosystem Assessed by Inter-simple Sequence Repeat (ISSR) Markers[J]. HEREDITAS, 2006, 28(8): 977-983.

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Genetic Diversity of Beauveria bassiana (Bals.) Vuill. in Forest Ecosystem Assessed by Inter-simple Sequence Repeat (ISSR) Markers

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