拟南芥AtWRKY25、AtWRKY26和AtWRKY33在非生物胁迫条件下的表达分析

doi:10.3724/SP.J.1005.2010.00848

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HEREDITAS ›› 2010, Vol. 32 ›› Issue (8): 848-856.doi: 10.3724/SP.J.1005.2010.00848

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Expression profiles of AtWRKY25, AtWRKY26 and AtWRKY33 under abiotic stresses

FU Qian-Tang^{1, 2}, YU Di-Qiu¹

1. Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China; 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received:2009-10-28 Revised:2010-01-14 Online:2010-08-20 Published:2010-08-23
Contact: YU Di-Qiu E-mail:ydq@xtbg.ac.cn

Abstract

Abstract: The transcription factor WRKY family is one type of key regulatory components of plant development and defense against stress factors. The expression profiles of three AtWRKY genes under abiotic stresses were analyzed by Northern blotting analysis. The expression of AtWRKY25, AtWRKY26, and AtWRKY33 changed during stress treatments including thermal factors, NaCl, abscisic acid (ABA) and osmotic stress, and significantly under NaCl and cold treatments, suggesting a specific role of the three AtWRKYs in adaptation to environmental stresses in plants. We also found that the three AtWRKY genes showed distinct expression patterns under thermal stresses. AtWRKY25 and AtWRKY26 were gradually induced during heat and cold treatments, whereas AtWRKY33 was suppressed by heat treatment and induced rapidly during cold stress, indicating that the three AtWRKYs may play different roles in response to temperature factors. In addition, we analyzed the sequence of the promoters with bioinformatics approach, and some cis-elements involved in abiotic stresses and hormonal responses were revealed. The results provided important information for studying biological functions of three AtWRKY genes.

Key words: Arabidopsis thaliana, AtWRKY geneIabiotic stressesIexpression profiles

FU Gan-Tang, TU Di-Qiu. Expression profiles of AtWRKY25, AtWRKY26 and AtWRKY33 under abiotic stresses[J]. HEREDITAS, 2010, 32(8): 848-856.

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Expression profiles of AtWRKY25, AtWRKY26 and AtWRKY33 under abiotic stresses

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