小麦转基因方法及其评述

doi:10.3724/SP.J.1005.2011.00422

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HEREDITAS ›› 2011, Vol. 33 ›› Issue (5): 422-430.doi: 10.3724/SP.J.1005.2011.00422

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Description and evaluation of transformation approaches used in wheat

YE Xing-Guo, CHEN Ming, DU Li-Pu, XU Hui-Jun

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2010-10-09 Revised:2010-12-04 Online:2011-05-20 Published:2011-05-25
Contact: 叶兴国 E-mail:yexg@mail.caas.net.cn

Abstract

Abstract: Genetic transformation is a valuable tool for direct crop improvement and functional genomics study. Unfortunately, wheat is considered as a recalcitrant plant to genetic transformation due to its low efficiency and genotype dependency. To overcome these problems, various transformation methods such as biolistic bombardment, Agrobacterium tume-faciens, pollen-tube pathway, ion implantation, laser microbeams puncture, treatment with polyethylene glycol and ultra-sonic wave, and electroporation have been reported in wheat using various types of explants including immature embryos, mature embryos, anthers derived calluses, inflorescences, apical meristems, and other floral organs. In this review, several major transformation approaches and their applications in wheat are reviewed, and potential strategies for the development of safe transgenic wheat plants are discussed. The objective of this review is to provide an update on current status of wheat transformation, and to stimulate further research for improving transformation efficiency in wheat.

Key words: wheat, genetic transformation

XIE Xin-Guo, CHEN Meng, DU Li-Pu, XU Hui-Jun. Description and evaluation of transformation approaches used in wheat[J]. HEREDITAS, 2011, 33(5): 422-430.

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Description and evaluation of transformation approaches used in wheat

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