东方山羊豆Cu/ZnSOD基因的克隆及表达分析

doi:10.3724/SP.J.1005.2012.00095

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HEREDITAS ›› 2012, Vol. 34 ›› Issue (1): 95-101.doi: 10.3724/SP.J.1005.2012.00095

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Cloning and expression analysis of Cu/ZnSOD gene from Galega orientalis L

LI Yu-Kun, WANG Xue-Min, GAO Hong-Wen, REN AI-Qin, WANG Zan, SUN Gui-Zhi

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2011-03-29 Revised:2011-10-15 Online:2012-01-20 Published:2012-01-25

Abstract

Abstract: SOD is an important enzyme which exists in eukaryote extensively and plays an essential role in stress-tolerance of higher plants. A cDNA of Cu/ZnSOD gene was cloned from Galega orientalis L. using rapid amplification of cDNA ends (RACE) method. The full-length of cDNA sequence is 935 bp, included a 600 bp open reading frame which encoded a 199-amino-acid polypeptide. The molecular weight of this protein was 20.35 kDa. The results of Real-Time PCR indicated that the expression level of Cu/ZnSOD gene was the highest in leaves, moderate in stems, and the least in roots. The expression of Cu/ZnSOD gene under stress of NaCl and PEG was up-regulated firstly and then declined. The expression level was significantly lower than the control after 24 h treated with NaCl. Abscisic acid downregulated the expression of Cu/ZnSOD gene. The result of subcellular localization indicated that Cu/ZnSOD was located in chloroplast. Gene Cu/ZnSOD mainly expressed in the green organs of G. orientalis and played a certain role in resisting osmotic stress.

Key words: Galega orientalis L, Cu/ZnSOD gene, cloning, real-time PCR, subcellular localization

LI Yu-Kun, WANG Xue-Min, GAO Hong-Wen, REN AI-Qin, WANG Zan, SUN Gui-Zhi. Cloning and expression analysis of Cu/ZnSOD gene from Galega orientalis L[J]. HEREDITAS, 2012, 34(1): 95-101.

References

[1] Alscher RG, Erturk N, Heath LS. Role of superoxide dis-mutases (SODs) in controlling oxidative stress in plants. J Exp Bot, 2002, 53(372): 1331-1341.
[2] Sen Raychaudhuri S, Deng XW. The role of superoxide dismutase in combating oxidative stress in higher plants. Bot Rev, 2000, 66(1): 89-98.
[3] Slooten L, van Montagu M, Inzé D. Manipulation of oxi-dative stress tolerance in transgenic plants. In: Lindsey K, ed. Transgenic Plant Research. Amsterdam: Harwood Academic Publishers, 1998: 241-262.
[4] Bannister JV, Bannister WH, Rotilio G. Aspects of the structure, function, and applications of superoxide dismutas. Cri Rev Biochem Mol Biol, 1987, 22(2): 111-180.
[5] 杜金芳, 曾勇庆, 陈伟, 崔景香, 陈其美, 杨伦, 胡艳霞. 猪CuZnSOD基因的克隆、表达及功能分析. 遗传, 2010, 32(10): 1037-1042.
[6] Song FN, Yang CP, Liu XM, Li GB. Effect of salt stress on activity of superoxide dismutase (SOD) in Ulmus pumila L. J Forestry Res, 2006, 17(1): 13-16.
[7] 郭丽红, 吴晓岚, 龚明. 谷胱甘肽还原酶和超氧化物歧化酶在玉米幼苗热激诱导的交叉适应中的作用. 植物生理学通讯, 2005, 41(4): 429-432.
[8] 冯昌军, 罗新义, 沙伟, 王凤国. 低温胁迫对苜蓿品种幼苗SOD、POD活性和脯氨酸含量的影响. 草业科学, 2005, 22(6): 29-32.
[9] Ronald E, Joseph A, John G. Cloning of cDNA for maize superoxide dismutase2 (SOD2). Proc Nat Acad Sci USA, 1987, 84(1): 179-183.
[10] Bowler C, Alliotte T, De Loose M, van Montagu M, Inzé D. The induction of manganese superoxide dismutase in response to stress in Nicotiana plumbaginifolia. EMBO J, 1989, 8(1): 31-38.
[11] Cannon RE, Scandalios JG. Two cDNAs encode two nearly identical Cu/Zn superoxide dismutase proteins in maize. Mol Gen Genet, 1989, 219(1): 1-8.
[12] Kaminaka H, Morita S, Yokoi H, Masumura T, Tanaka K. Molecular cloning and characterization of a cDNA for plastidic copper/zinc-superoxide dismutase in rice (Oryza sativa L.). Plant Cell Physiol, 1997, 38(1): 65-69.
[13] Perl-Treves R, Nacmias B, Aviv D, Zeelon EP, Galun E. Isolation of two cDNA clones from tomato containing two different superoxide dismutase sequences. Plant Mol Biol, 1988, 11(5): 609-623.
[14] Sakamoto A, Ohsuga H, Tanaka K. Nucleotide sequences of two cDNA clones encoding different Cu/Zn-superoxide dismutases expressed in developing rice seed (Oryza sativa L.). Plant Mol Biol, 1992, 19(2): 323-327.
[15] Bueno P, Piqueras A, Kurepa J, Savouré A, Verbruggen N, van Montagu M, Inzé D. Expression of antioxidant en-zymes in response to abscisic acid and high osmoticum in tobacco BY-2 cell cultures. Plant Sci, 1998, 138(1): 27-34.
[16] Hernández-Nistal J, Dopico B, Labrador E. Cold and salt stress regulates the expression and activity of a chickpea cytosolic Cu/Zn superoxide dismutase. Plant Sci, 2002, 163(3): 507-514.
[17] McKersie BD, Chen Y, De Beus M, Bowley SR, Bowler C, Inze D, D'Halluin K, Botterman J. Superoxide dismutase enhances tolerance of freezing stress in transgenic alfalfa (Medicago sativa L.). Plant Physiol, 1993, 103(4): 1155-1163.
[18] McKersie BD, Murnaghan J, Jones KS, Bowley SR. Iron-superoxide dismutase expression in transgenic alfalfa increases winter survival without a detectable increase in photosynthetic oxidative stress tolerance. Plant Physiol, 2000, 122(4): 1427-1438.
[19] van Camp W, Capiau K, van Montagu M, Inze D, Slooten L. Enhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts. Plant Physiol, 1996, 112(4): 1703-1714.
[20] Dorofejuk MT, Dorofejuk VF, Addrusewitsch VT. Cultivation of eastern goat's rue (Galega orientalis Lam) as a perennial fodder plant on turf-podzol soils in the Republic of Belarus. M Archives of Agron Soil Sci, 1999, 44(2): 131-148.
[21] 刘法涛, 杨志忠. 优良豆科牧草——东方山羊豆. 草食家畜, 2000, (2): 42.
[22] 张自和, 于应文. 东方山羊豆在俄罗斯的研究和应用. 草业科学, 2006, 23(1): 26-33.
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Cloning and expression analysis of Cu/ZnSOD gene from Galega orientalis L

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