棉花Trihelix转录因子GhGT29基因的克隆及功能分析

doi:10.16288/j.yczz.15-320

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HEREDITAS(Beijing) ›› 2015, Vol. 37 ›› Issue (12): 1218-1227.doi: 10.16288/j.yczz.15-320

• Research Articles • Previous Articles Next Articles

Cloning and functional analysis of the cotton Trihelix transcription factor GhGT29

Yue Li¹, Xiaodong Liu¹, Yongmei Dong², Zongming Xie², Shouyi Chen³

1. Key Lab of Agro-Biotechnology, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China;
2. Biotechnology Research Institute, Xinjiang Academy of Agricultural and Reclamation Science/Xinjiang Production and Construction Group Key Laboratory of Crop Germplasm Enhancement and Gene Resources Utilization, Shihezi 832000, China;
3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences/National Key Laboratory of Plant Genomics, Beijing 100101,China

Received:2015-07-15 Online:2015-12-20 Published:2015-09-28

Abstract

Abstract: Trihelix transcription factors are important proteins involved in response to abiotic stresses in plants. Understanding the molecular mechanisms of Trihelix in cottons will lay the foundation to improve stress tolerance by gene engineering. In this study, a gene encoding Trihelix transcription factor was isolated in upland cottons using reverse transcription PCR according to bioinformatic analysis. The gene was named as GhGT29 (GenBank accession No. JQ013097), which was 1 092 bp, contained a 1 089 bp open reading frame and encoded a protein of 363 amino acids with a predicted molecular weight of 40.9 kDa and a isoelectric point of 5.45. SMART analysis showed GhGT29 contained one typical SANT motif. Phylogenetic analysis showed that GhGT29 belonged to the SH4 subfamily of the Trihelix family and was most closely related to AtSH4-like1 and AtSH4-like2. Quantitative real-time PCR (qRT-PCR) analysis revealed that GhGT29 was induced by high salt, drought, cold and abscisic acid. The expression profile also revealed that GhGT29 was constitutively expressed in all tested tissues, such as roots, stems, leaves, flowers, ovules (0 DPA) and fibers (12 DPA). The expression level of GhGT29 was the highest in flowers and the lowest in stems. Using the Arabidopsis protoplasts assay system, we found that the GhGT29 protein was located in cell nuclei and had trans-activation activity. These results revealed that GhGT29 might be involved in the regulation of stress resistance-related genes in stress signaling pathways in upland cottons.

Key words: cotton, Trihelix transcription factor, abiotic stress, subcellular localization, trans-activation activity

Yue Li, Xiaodong Liu, Yongmei Dong, Zongming Xie, Shouyi Chen. Cloning and functional analysis of the cotton Trihelix transcription factor GhGT29[J]. HEREDITAS(Beijing), 2015, 37(12): 1218-1227.

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Cloning and functional analysis of the cotton Trihelix transcription factor GhGT29

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