OsGA3ox通过合成不同活性GA调控水稻育性及株高

doi:10.16288/j.yczz.23-058

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Hereditas(Beijing) ›› 2023, Vol. 45 ›› Issue (9): 845-855.doi: 10.16288/j.yczz.23-058

• Research Article • Previous Articles

OsGA3ox genes regulate rice fertility and plant height by synthesizing diverse active GA

Xiaohua Hao^1,²(), Shuang Hu², Dan Zhao², Lianfu Tian², Zijing Xie², Sha Wu², Wenli Hu², Han Lei², Dongping Li²()

1. Plant Product Deep Processing Technology Innovation Team, College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, China
2. College of Life Science, Hunan Normal University, Changsha 410081, China

Received:2023-03-15 Revised:2023-06-14 Online:2023-09-20 Published:2023-07-19
Contact: Dongping Li E-mail:14771417@qq.com;dli@hunnu.edu.cn
Supported by:
National Natural Science Foundation of China(32071930);Hunan Province Natural Science Foundation Project(2022JJ30421);Hunan Province Natural Science Foundation Project(2021JJ30445);Changsha City Natural Science Foundation Project(kq2014076)

Abstract

Abstract:

Gibberellin (GA) is an important hormone, which is involved in regulating various growth and development. GA biosynthesis pathway and synthetase have been basically clarified. Gibberellin 3β hydroxylase (GA3ox) is the key enzyme for the synthesis of various active GA. There are two GA3ox genes (OsGA3ox1 and OsGA3ox2) in rice, and their physiological functions have been preliminarily studied. However, it is not clear how they work together to synthesize active GA to regulate rice development. In this study, the knockout mutants ga3ox1 and ga3ox2 were obtained by CRISPR/Cas9 technology. The pollen fertility of ga3ox1 decreased significantly, while the plant height of ga3ox2 decreased significantly. It shows that OsGA3ox1 is necessary for normal pollen development, while OsGA3ox2 is necessary for stem and leaf elongation. Tissue expression analysis showed that OsGA3ox1 was mainly expressed in unopened flowers, while OsGA3ox2 was mainly expressed in unexpanded leaves. The GA in different tissues of wild type (WT), and two ga3ox mutants were detected. It was found that pollen fertility is most closely related to the content of GA₇, and plant height is most closely related to the content of GA₁. It was found that OsGA3ox1 catalyzes GA₉ to GA₇ in flowers, which is closely related to pollen fertility; OsGA3ox2 catalyzes the GA₂₀ to GA₁ in unexpanded leaves, thereby regulating plant height; OsGA3ox1 catalyzes the GA₁₉ to GA₂₀ in roots, regulating the generation of GA₃. OsGA3ox1 and OsGA3ox2 respond to developmental and environmental signals, and cooperate to synthesize endogenous GA in different tissues to regulate rice development. This study provides a reference for clarifying its role in GA biosynthesis pathway and further understanding the function of OsGA3ox.

Key words: Oryza sativa L., gibberellin (GA), OsGA3ox, functional analysis

Xiaohua Hao, Shuang Hu, Dan Zhao, Lianfu Tian, Zijing Xie, Sha Wu, Wenli Hu, Han Lei, Dongping Li. OsGA3ox genes regulate rice fertility and plant height by synthesizing diverse active GA[J]. Hereditas(Beijing), 2023, 45(9): 845-855.

Figures/Tables 7

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引物名称	引物序列(5′→3′)	用途
OsGA3ox1-RT-PCR	F：CGAGACCGAGCGGAAGA	半定量RT-PCR和实时荧光定量PCR
OsGA3ox1-RT-PCR	R：GCCGACGACGACGATGA	半定量RT-PCR和实时荧光定量PCR
OsGA3ox2-RT-PCR	F：TTCTCCAAGCTCATGTGGTC	半定量RT-PCR和实时荧光定量PCR
OsGA3ox2-RT-PCR	R：GCATCTCCTTGTGAAACTC	半定量RT-PCR和实时荧光定量PCR
OsActin2-RT-PCR	F：ATGTGCCAGCTATGTATGTC	半定量RT-PCR和实时荧光定量PCR
OsActin2-RT-PCR	R：CGTTCAGCAGTGGTAGTGA	半定量RT-PCR和实时荧光定量PCR
OsGA3ox1-Cas9-1	F：GCCGGAGTCGCACGTGTGGA	构建CRISPR/Cas9基因编辑载体
OsGA3ox1-Cas9-1	R：TCCACACGTGCGACTCCGGC	构建CRISPR/Cas9基因编辑载体
OsGA3ox1-Cas9-2	F：CGATGAGAGCTCTGGGCGAG	构建CRISPR/Cas9基因编辑载体
OsGA3ox1-Cas9-2	R：CTCGCCCAGAGCTCTCATCG	构建CRISPR/Cas9基因编辑载体
OsGA3ox2-Cas9-1	F：GCTCTGCTTCGACTTCCGGG	构建CRISPR/Cas9基因编辑载体
	R：CCCGGAAGTCGAAGCAGAGC	构建CRISPR/Cas9基因编辑载体
OsGA3ox2-Cas9-2	F：GAGAAGATGCGCGCCGTCCG	构建CRISPR/Cas9基因编辑载体
OsGA3ox2-Cas9-2	R：CGGACGGCGCGCATCTTCTC	构建CRISPR/Cas9基因编辑载体
OsGA3ox1-GUS	F：GGTTTTCATGCCATGCCAAT	构建GUS表达载体
OsGA3ox1-GUS	R：GCATGAACTCGTTGGCTA	构建GUS表达载体
OsGA3ox2-GUS	F：ATAGTCCTCGGCAAGAAG	构建GUS表达载体
OsGA3ox2-GUS	R：CGACGACGACGACGAT	构建GUS表达载体

OsGA3ox genes regulate rice fertility and plant height by synthesizing diverse active GA

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