水稻突触融合相关蛋白基因(OsKNOLLE)在酵母中的异源表达及在非生物胁迫中的作用初探

doi:10.3724/SP.J.1005.2011.01251

[an error occurred while processing this directive]

HEREDITAS ›› 2011, Vol. 33 ›› Issue (11): 1251-1257.doi: 10.3724/SP.J.1005.2011.01251

• en • Previous Articles Next Articles

Heterologous expression of a rice syntaxin-related protein KNOLLE gene (OsKNOLLE) in yeast and its functional analysis in the role of abiotic stress

WANG Fei-Juan, ZHU Cheng

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China

Received:2011-03-17 Revised:2011-06-18 Online:2011-11-20 Published:2011-11-25
Contact: ZHU Cheng E-mail:pzhuch@zju.edu.cn

Abstract

Abstract: Syntaxin-related protein KNOLLE, a multifunctional protein family belonging to the SNARE superfamily, plays an important role in many physiological processes in plants. In order to understand the function of the syntaxin-related protein KNOLLE (OsKNOLLE) in rice (Oryza sativa), the CDS sequence of OsKNOLLE gene isolated from a japonica rice cultivar “Zhonghua 11” was fused into an expression vector pYX212 and transformed into the S. cerevisiae strain BY4741 by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. The transformants with OsKNOLLE showed better survival abilities than the transformants with the empty vectors based on their phenotypes in responses to different abiotic stresses such as salt, Cu²⁺, H₂O₂, Cd²⁺, and Hg²⁺. These data suggests that OsKNOLLE plays a crucial role in responses to abiotic stresses. This experimental system sets up a method for studying functions of the OsKNOLLE gene in the future and clarifies the relationship between OsKNOLLE and abiotic stresses.

Key words: rice, syntaxin-related protein KNOLLE, heteroexpression, abiotic stress, functional analysis

CLC Number:

WANG Fei-Juan, ZHU Cheng. Heterologous expression of a rice syntaxin-related protein KNOLLE gene (OsKNOLLE) in yeast and its functional analysis in the role of abiotic stress[J]. HEREDITAS, 2011, 33(11): 1251-1257.

References

[1] Baker AJM. Metal tolerance. New Phytol, 1987, 106(1): 93-111.
[2] Clemens S. Molecular mechanisms of plant metal tolerance and homeostasis. Planta, 2001, 212(4): 475-486.
[3] Hall JL. Cellular mechanisms for heavy metal detoxification and tolerance. J Exp Bot, 2002, 53(366): 1-11.
[4] Feng H, Chen C, Wang YQ, Qiu JL, Chu CC, Du XH. Plant SNAREs and their biological functions. Hereditas, 2009, 31(5): 471-478.
[5] Lipka V, Dittgen J, Bednarek P, Bhat R, Wiermer M, Stein M, Landtag J, Brandt W, Rosahl S, Scheel D, Llorente F, Molina A, Parker J, Somerville S, Schulze-Lefert P. Pre- and postinvasion defenses both contribute to nonhost resistance in Arabidopsis. Science, 2005, 310(5751): 1180-1183.
[6] Bao YM, Wang JF, Huang J, Zhang HS. Cloning and characterization of three genes encoding Qb-SNARE proteins in rice. Mol Genet Genomics, 2008, 279(3): 291-301.
[7] Geelen D, Leyman B, Batoko H, Di Sansabastiano GP, Moore I, Blatt MR. The abscisic acid-related SNARE homolog NtSyr1 contributes to secretion and growth: Evidence from competition with its cytosolic domain. Plant Cell, 2002, 14(2): 387-406.
[8] Collins NC, Thordal-Christensen H, Lipka V, Bau S, Kombrink E, Qiu JL, Hückelhoven R, Stein M, Freialdenhoven A, Somerville SC, Schulze-Lefert P. SNARE- protein-mediated disease resistance at the plant cell wall. Nature, 2003, 425(6961): 973-977.
[9] Leshem Y, Golani Y, Kaye Y, Levine A. Reduced expression of the v-SNAREs AtVAMP71/AtVAMP7C gene family in Arabidopsis reduces drought tolerance by suppression of abscisic acid-dependent stomatal closure. J Exp Bot, 2010, 61(10): 2615-2622.
[10] Benschop JJ, Mohammed S, O’Flaherty M, Heck AJR, Slijper M, Menke FLH. Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis. Mol Cell Proteomics, 2007, 6(7): 1198-1214.
[11] Bao YM, Liu YH, Xu DQ, Huang J, Wang ZF, Wang JF, Zhang HS. Preparation, characterization and application of rice Qb-SNARE protein OsNPSN11 polyclonal antibody. Hereditas, 2010, 32(9): 961-965.
[12] Wang FJ, Zeng B, Sun ZX, Zhu C. Relationship between proline and Hg²⁺-induced oxidative stress in a tolerant rice mutant. Arch Environ Contam Toxicol, 2009, 56(4): 723-731.
[13] Gietz RD, Woods RA. Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Method Enzymol, 2002, 350: 87-96.
[14] Yan SP, Tang ZC, Su WA, Sun WN. Proteomic analysis of salt stress-responsive proteins in rice root. Proteomics, 2005, 5(1): 235-244.
[15] Chitteti BR, Peng HH. Proteome and phosphoproteome differential expression under salinity stress in rice (Oryza sativa) roots. J Proteome Res, 2007, 6(5): 1718-1727.
[16] Shri M, Kumar S, Chakrabarty D, Trivedi PK, Mallick S, Misra P, Shukla D, Mishra S, Srivastava S, Tripathi RD, Tuli R. Effect of arsenic on growth, oxidative stress, and antioxidant system in rice seedlings. Ecotox Environ Safe, 2009, 72(4): 1102-1110.
[17] Jürgens G. Membrane trafficking in plants. Annu Rev Cell Dev Biol, 2004, 20(1): 481-504.
[18] Sokolovski S, Hills A, Gay RA, Blatt MR. Functional interaction of the SNARE protein NtSyp121 in Ca²⁺ channel gating, Ca²⁺ transients and ABA signalling of stomatal guard cells. Mol Plant, 2008, 1(2): 347-358.
[19] Leshem Y, Melamed-Book N, Cagnac O, Ronen G, Nishri Y, Solomon M, Cohen G, Levine A. Suppression of Arabidopsis vesicle-SNARE expression inhibited fusion of H₂O₂-containing vesicles with tonoplast and increased salt tolerance. Proc Natl Acad Sci USA, 2006, 103(47): 18008-18013.
[20] Mazel A, Leshem Y, Tiwari BS, Levine A. Induction of salt and osmotic stress tolerance by overexpression of an intracellular vesicle traffick

Metrics

Comments

www.chinagene.cn
备案号：京ICP备09063187号

Heterologous expression of a rice syntaxin-related protein KNOLLE gene (OsKNOLLE) in yeast and its functional analysis in the role of abiotic stress

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Zhong Bian, Dongping Cao, Wenshu Zhuang, Shuwei Zhang, Qiaoquan Liu, Lin Zhang. Revelation of rice molecular design breeding: the blend of tradition and modernity [J]. Hereditas(Beijing), 2023, 45(9): 718-740.
[2]	Xiangdong Liu, Jinwen Wu, Zijun Lu, Muhammad Qasim Shahid. Autotetraploid rice: challenges and opportunities [J]. Hereditas(Beijing), 2023, 45(9): 781-792.
[3]	Xiaohua Hao, Shuang Hu, Dan Zhao, Lianfu Tian, Zijing Xie, Sha Wu, Wenli Hu, Han Lei, Dongping Li. OsGA3ox genes regulate rice fertility and plant height by synthesizing diverse active GA [J]. Hereditas(Beijing), 2023, 45(9): 845-855.
[4]	Zhenwu Zheng, Hongyuan Zhao, Xiaoya Liang, Yijun Wang, Chihang Wang, Gaoyan Gong, Jinyan Huang, Guiquan Zhang, Shaokui Wang, Zupei Liu. qGL3.4 controls kernel size and plant architecture in rice [J]. Hereditas(Beijing), 2023, 45(9): 835-844.
[5]	Mingjiang Chen, Guifu Liu, Yeqing Xiao, Hong Yu, Jiayang Li. Breeding of ZhongKeFaZaoGeng1 by molecular design [J]. Hereditas(Beijing), 2023, 45(9): 829-834.
[6]	Xiaoping Lian, Guangfu Huang, Yujiao Zhang, Jing Zhang, Fengyi Hu, Shilai Zhang. The discovery and utilization of favorable genes in Oryza longistaminata [J]. Hereditas(Beijing), 2023, 45(9): 765-780.
[7]	Yongqiang Liu, Weiwei Li, Xinyu Liu, Chengcai Chu. Molecular mechanism of tillering response to nitrogen in rice [J]. Hereditas(Beijing), 2023, 45(5): 367-378.
[8]	Rongrong Mu, Qingqing Niu, Yuqiang Sun, Jun Mei, Meng Miao. Cloning and characterization of the MYB transcription factor gene GhTT2 in Gossypium hirsutum [J]. Hereditas(Beijing), 2022, 44(8): 720-728.
[9]	Shan Li, Yunzhi Huang, Xueying Liu, Xiangdong Fu. Genetic improvement of nitrogen use efficiency in crops [J]. Hereditas(Beijing), 2021, 43(7): 629-641.
[10]	Cailin Wang, Yadong Zhang, Chunfang Zhao, Xiaodong Wei, Shu Yao, Lihui Zhou, Zhen Zhu, Tao Chen, Qingyong Zhao, Ling Zhao, Kai Lu, Wenhua Liang. Inheritance and breeding of japonica rice with good eating quality in Jiangsu province [J]. Hereditas(Beijing), 2021, 43(5): 442-458.
[11]	Changquan Zhang, Linhao Feng, Minghong Gu, Qiaoquan Liu. Progress on inheritance and gene cloning for rice grain quality in Jiangsu province [J]. Hereditas(Beijing), 2021, 43(5): 425-441.
[12]	Hang Dai, Yan Li, Shunchun Liu, Lei Lin, Juanyan Wu, Zhijie Zhang, Qichun Peng, Nan Li, Xiangqian Zhang. Effect of extensin-like OsPEX1 on pollen fertility in rice [J]. Hereditas(Beijing), 2021, 43(3): 271-279.
[13]	Lingyue Yan, Haojian Zhang, Yuqing Zheng, Yunqi Cong, Citao Liu, Fan Fan, Cheng Zheng, Guilong Yuan, Gen Pan, Dingyang Yuan, Meijuan Duan. Transcription factor OsMADS25 improves rice tolerance to cold stress [J]. Hereditas(Beijing), 2021, 43(11): 1078-1087.
[14]	Yali Hu, Rui Dai, Yongxin Liu, Jingying Zhang, Bin Hu, Chengcai Chu, Huaibo Yuan, Yang Bai. Analysis of rice root bacterial microbiota of Nipponbare and IR24 [J]. Hereditas(Beijing), 2020, 42(5): 506-518.
[15]	Guiquan Zhang. The platform of breeding by design based on the SSSL library in rice [J]. Hereditas(Beijing), 2019, 41(8): 754-760.