荧光RNA及其生物传感技术研究进展

doi:10.16288/j.yczz.23-306

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Hereditas(Beijing) ›› 2024, Vol. 46 ›› Issue (2): 92-108.doi: 10.16288/j.yczz.23-306

• Invited Review • Previous Articles Next Articles

Progress on fluorescent RNA and fluorescent RNA-based biosensing technology

Fangting Zuo^1,^2,³(), Yaqiang Zhang^1,^2,³, Huimin Yang^1,², Yi Yang^1,²(), Xianjun Chen^1,²()

1. Interdisciplinary Research Center of Optogenetics and Synthetic Biology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
2. Shanghai Advanced Research Base of Cell Metabolism Genetics, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
3. School of Bioengineering, East China University of Science and Technology, Shanghai 200237, China

Received:2023-12-12 Revised:2024-01-25 Online:2024-02-20 Published:2024-01-29
Contact: Yi Yang,Xianjun Chen E-mail:ftzuo@ecust.edu.cn;yiyang@ecust.edu.cn;xianjunchen@ecust.edu.cn
Supported by:
National Natural Science Foundation of China(32121005);National Natural Science Foundation of China(32150028);National Natural Science Foundation of China(21937004);National Natural Science Foundation of China(91857202);National Natural Science Foundation of China(32250009);National Key Research and Development Program of China(2022YFC3400100)

Abstract

Abstract:

Fluorescent RNA is a kind of emerging RNA labeling technique that can be used for in situ labeling and imaging of RNA in live cells, which plays an important role in understanding the function and regulation mechanism of RNA. Biosensing technology based on fluorescent RNA can be applied in dynamic detection of small molecule metabolites and proteins in real time, offering valuable tools for basic life science research and biomedical sensing technology development. In this review, we introduce the development of genetically encoded fluorescent RNA, and the application of fluorescent RNA in RNA imaging and biosensing technology based on fluorescent RNA in biosensing in live cell. Meanwhile, we discuss the direction and challenge of future development of fluorescent RNA technology to provide valuable insights for further development and application of this technology in relevant fields.

Key words: fluorescent RNA, fluorescent labeling, RNA imaging, biosensing

Fangting Zuo, Yaqiang Zhang, Huimin Yang, Yi Yang, Xianjun Chen. Progress on fluorescent RNA and fluorescent RNA-based biosensing technology[J]. Hereditas(Beijing), 2024, 46(2): 92-108.

Figures/Tables 8

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荧光RNA	光谱^a	亲和力^b	激活倍数^c	体外亮度^d	细胞亮度	超分辨	温度稳定性^e	文献
Pepper-HBC系列	青到红色	3.5 ^f	3595	128	高	NA	55	[59]
Pepper-HBC系列	青到红色	6.1 ^g	12600	173	高	SIM	66	[59]
Broccoli-BI	470/505	51	1390	67	较高	NA	42	[60]
RhoBAST-TMR-DN	564/590	15	26	163	高	SMLM	79	[62]
biRhoBAST-TMR₂	564/590	0.04	55	458	高	NA	56	[63]
RhoBAST-SpyRho	562/581	34	60	184	高	SMLM&STED	NA	[64]
Clivia-NBSI系列	黄到红色	55 ^h	286	47	较高	SIM	44	[65]

Progress on fluorescent RNA and fluorescent RNA-based biosensing technology

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