陆地棉超矮杆突变体基因的初步定位

doi:10.3724/SP.J.1005.2011.01393

[an error occurred while processing this directive]

HEREDITAS ›› 2011, Vol. 33 ›› Issue (12): 1393-1397.doi: 10.3724/SP.J.1005.2011.01393

• en • Previous Articles Next Articles

Gene mapping of an ultra-dwarf mutant in upland cotton

JING Chao¹, MA Xiao-Jie², DI Jia-Chun², CHEN Xu-Sheng²

1. Agronomy College of Nanjing Agricultural University, Nanjing 210095, China 2. Key Laboratory of Cotton and Rape in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Jiangsu Academy of Agri-cultural Sciences, Nanjing 210014, China

Received:2011-04-03 Revised:2011-10-21 Online:2011-12-20 Published:2011-12-25

Abstract

Abstract: A ultra-dwarf mutant was newly found in upland cotton (Gossypium hirsutum L.). This mutant was controlled by single recessive gene du. Seventy pairs of polymorphic primers were selected from 1350 primers, which covered all the identified chromosomes by screening in parents and near-isogenic lines. Gene du was mapped using an F₂ population derived from intraspecific crosses between “Ultra-dwarf 1” and “Xinluzao 16”. Thirty-six primers were distributed in eight linkage groups, and du was linked to LG01. Seven co-dominant markers linked to du were NAU2679, NAU2749, NAU905, NAU2838, NAU5373, NAU2238, and NAU4946. Based on the known genetic map of tetraploid cotton, the markers NAU4946, NAU2238, NAU905, NAU5373, and NAU2679 were located on chromosome 6, and the target gene du was located between NAU2238 and NAU4946 with the genetic distances of 3.3 cM and 1.4 cM, respectively. Hence, gene du was located on chromosome 6.

Key words: upland cotton (G. hirsutum L.), ultra-dwarf mutant, gene location, SSR

JING Chao, MA Xiao-Jie, DI Jia-Chun, CHEN Xu-Sheng. Gene mapping of an ultra-dwarf mutant in upland cotton[J]. HEREDITAS, 2011, 33(12): 1393-1397.

References

[1] 嵇怡, 缪曼珉, 陈学好. 植物矮生性状的分子遗传研究进展. 分子植物育种, 2006, 4(6): 753-77l.
[2] Khush GS. Green revolution: preparing for the 21st century. Genome, 1999, 42(4): 646-655.
[3] Korzun V, Röder M, Worland AJ, Börner A. Intrachromosomal mapping of genes for dwarfing (Rht12) and vernalization response (Vrn1) in wheat by using RFLP and microsatellite markers. Plant Breed, 1997, 116(3): 227-232.
[4] Korzun V, Roder MS, Ganal MW, Worland AJ, Law CN. Genetic analysis of the dwarfing gene (Rht8) in wheat, Part I. Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat (Triticum aestivum L.). Theor Appl Genet, 1998, 96(8): 1104-1109.
[5] 张京. 大麦矮杆基因uz的SSR标记. 作物学报, 2003, 29(4): 637-640.
[6] 车俐晓, 陈杰, 展霞, 桑燕, 刘敏, 刘保申. 水稻矮秆突变体sde(t)的遗传分析与基因初步定位. 分子植物育种, 2008, 6(4): 760-764.
[7] 袁运动, 程祝宽, 刘保申, 李传友. 水稻矮秆突变体ipd1的遗传分析及其基因精细定位. 山东农业大学学报 (自然科学版), 2008, 40(1): 1-6.
[8] 梅德圣, 王汉中, 李云昌, 胡琼, 李英德, 徐育松. 甘蓝型油菜矮杆突变材料99CDAM的发现及遗传分析. 遗传, 2006, 28(7): 851-857.
[9] 姜亮, 郭龙彪, 姜华, 曾大力, 胡江, 胡立文, 高振宇, 钱前. 一个矮杆叶尖枯水稻突变体的遗传分析和精细定位. 遗传学报, 2008, 35(12): 715-721.
[10] Harland SC. On the genetics of crinkled dwarf rogues in Sea Island cotton. West Ind Bull, 1918, 16(1): 82.
[11] Harland SC. The genetics of cotton. Part IX. Further experience on the inheritance of the crinkled dwarf mutant of G. barbadense. L. in interspecific cross and their bearing on Fisher theory of dominance. J Genet, 1933, 28(2): 315-325.
[12] Hutchinson JB, Ghose RU. On the occurrence of “crinkled dwarf” in Gossypium hirsutum. L. J Genet, 1937, 34(3): 437-446.
[13] Abzalov MF, Zhumaev FK. Revelation in the cotton plant of chromosomes responsible for the regulation of plant growth. Chem Natl Compounds, 1995, 31(1): 111-113.
[14] 何鉴星, 孙传渭, 姜茹琴, 王志龙, 张欣雪, 李爱国, 李赠书, 赵国忠. 棉花矮化突变体的遗传分析. 遗传学报, 1996, 23(5): 397-402.
[15] 陈旭升. 棉花矮杆突变体. 中国棉花, 2004, 31(1): 269.
[16] 陈旭升, 狄佳春, 许乃银, 肖松华, 刘剑光. 陆地棉超矮杆突变性状质量遗传规律分析. 遗传, 2007, 29(4): 471-474.
[17] Paterson AH, Brubaker C, Wendel JF. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis. Plant Mol Biol Rep, 1993, 11(2): 122-127.
[18] 张天真, 袁有禄, 郭旺珍, Yu J, Kohel RJ. 棉花高强纤维QTLs的微卫星标记筛选. 中国农业科学, 2001, 34(4): 363-366.
[19] 张军, 武耀廷, 郭旺珍, 张天真. 棉花微卫星标记的PAGE/银染快速检测. 棉花学报, 2000, 12(5): 267-269.
[20] Michelmore RW, Paran L, Kesseli RV. Identification of markers linked to disease-resistance genes by bulked seg-regant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88(21): 9828-9832.
[21] Guo WZ, Cai CP, Wang CB, Han ZG, Song XL, Wang K, Niu XW, Wang C, Lu KY, Shi B, Zhang TZ. A microsa-tellite-based, gene-rich linkage map reveals genome structure, function and evolution in Gossypium. Ge-netics, 2007, 176(1): 527-541.

Metrics

Comments

www.chinagene.cn
备案号：京ICP备09063187号

Gene mapping of an ultra-dwarf mutant in upland cotton

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Wanjin Xing, Morigen. Cultivating the scientific research ability of undergraduate students in teaching of genetics [J]. Hereditas(Beijing), 2016, 38(11): 1030-1038.
[2]	Gaofei Sun,Shoupu He,Zhaoe Pan,Xiongming Du. Homologous simple sequence repeats (SSRs) analysis in tetraploid (AD₁) and diploid (A₂, D₅) genomes of Gossypium [J]. HEREDITAS(Beijing), 2015, 37(2): 192-203.
[3]	Qiaoling Zhao, Wenbo Wang, Anli Chen, Zhiyong Qiu, Dingguo Xia, Heying Qian, Xingjia Shen. Genetic analysis and gene mapping of two novel quail-like mutants from the silkworm (Bombyx mori) [J]. HEREDITAS, 2014, 36(4): 369-375.
[4]	Yong Ren, Shengrong Li, Jianming Luo, Zhonghu He, Xiaoying Du, Qiang Zhou, Yuanjiang He, Yuming Wei, Youliang Zheng. Frequency and contribution of specific genetic loci transferred from wheat cultivar Mianmai 37 to its derivatives [J]. HEREDITAS, 2014, 36(2): 145-151.
[5]	Fengli Li, Jiachun Di, Liang Zhao, Xusheng Chen. Mapping of a new wrinkled leaf (wr3) gene in upland cotton [J]. HEREDITAS(Beijing), 2014, 36(12): 1256-1260.
[6]	Yonghu Zhang, Haifeng Yu, Jianhua Hou, Suping Li, Pin Lv, Zhixian Yu. Construction of a genetic map of sunflower using a population of recombinant inbred lines (RILs) [J]. HEREDITAS(Beijing), 2014, 36(10): 1036-1042.
[7]	WEN Ying LU Xiao-Ping REN Rui MI Fu-Gui HAN Ping-An XUE Chun-Lei. Development of EST-SSR marker and genetic diversity analysis in Sorghum bicolor×Sorghum sudanenes [J]. HEREDITAS, 2013, 35(2): 225-232.
[8]	YAN Hong-Mei, DONG Chao, ZHANG En-Lai, TANG Cui-Feng, A Xin-Xiang, YANG Wen-Yi, YANG Ya-Yun, ZHANG Fei-Fei, XU Fu-Rong. Analysis of genetic variation in rice paddy landraces across 30 years as revealed by microsatellite DNA markers [J]. HEREDITAS, 2012, 34(1): 87-94.
[9]	WANG Zun-Bao, DIAO Zong-Qing, TU Feng, TUN Hong-Bin, BAN Qian, LIANG Yao-Wei, ZHENG Wei. The gene ontology and electro localization of ovine skin derived EST-SSR markers [J]. HEREDITAS, 2011, 33(7): 731-737.
[10]	LI Lin, LIANG Hong-Wei, LI Zhong, LUO Xiang-Zhong, HE Guang-Fu, ZHANG Zhi-Wei, SHU Yuan-Yuan, JU Gui-Wei. Sequence and phylogeny analysis of the complete mitochondrial genome of Pelteobagrus vachelli [J]. HEREDITAS, 2011, 33(6): 627-635.
[11]	REN Yong, LI Sheng-Rong, LI Jun, ZHOU Qiang, DU Xiao-Ying, LI Tai-Jun, YANG Wu-Yun, ZHENG You-Liang. Genetic analysis and molecular mapping of stripe rust resistance gene in a restore line of Thermo-Photo sensitive hybrid wheat MR168 [J]. HEREDITAS, 2011, 33(11): 1263-1270.
[12]	FENG Su-Ping, LI Wei-Guo, XU Fei, WANG Jing-Yi, WU Yao-Ting. Construction of genetic linkage map for rubber tree (Hevea brasiliensis)based on SSR markers [J]. HEREDITAS, 2010, 32(8): 857-863.
[13]	. SSR linkage map construction and QTL mapping for leaf area in maize [J]. HEREDITAS, 2010, 32(6): 625-631.
[14]	Zhu Qi-wei, LI Huo-Gen. Comparison of genetic diversity among five mating types of Liriodendron revealed by SSR markers [J]. HEREDITAS, 2010, 32(2): 183-188.
[15]	HAN Nan, NIU Bao-Long, WANG Hai-Long, PENG Li, MENG Zhi-Qi. Mapping of the lethal genes in the sex-linkaged balanced lethal silk-worm Bombyx mori using SSR markers [J]. HEREDITAS, 2010, 32(12): 1269-1274.