蚂蚁抗菌肽Ponericin W1对植物病原菌的体外抑菌活性及其转基因拟南芥的抗病性

doi:10.3724/SP.J.1005.2013.01023

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HEREDITAS ›› 2013, Vol. 35 ›› Issue (8): 1023-1029.doi: 10.3724/SP.J.1005.2013.01023

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Antimicrobial activities of ant Ponericin W1 against plant pathogens in vitro and the disease resistance in its transgenic Arabidopsis

CHEN Yong-Fang¹, SUN Peng-Wei^1,2, TANG Ding-Zhong¹

1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2013-04-01 Revised:2013-05-08 Online:2013-08-20 Published:2013-08-25

Abstract

Abstract:

The antimicrobial peptides (AMPs) exhibit a broad antimicrobial spectrum. The application of AMPs from non-plant organisms attracts considerable attention in plant disease resistance engineering. Ponericin W1, isolated from the venom of ant (Pachycondyla goeldii), shows antimicrobial activities against Gram-positive bacteria, Gram-negative bacteria and the budding yeast (Saccharomyces cerevisiae); however, it is not clear whether Ponericin W1 is effective against plant pathogens. The results of this study indicated synthesized Ponericin W1 inhibited mycelial growth of Magnaporthe oryzae and Botrytis cinerea, as well as hyphal growth and spore production of Fusarium graminearum. Besides, Ponericin W1 exhibited antibacterial activities against Pseudomonas syringae pv. tomato and Xanthomonas oryzae pv. oryzae. After codon optimization, Ponericin W1 gene was constructed into plant expression vector, and transformed into Arabidopsis thaliana by floral dip method. The Ponericin W1 was located in intercellular space of the transgenic plants as expected. Compared with the wild-type plants, there were ungerminated spores and less hyphal, conidia on the leaves of transgenic plants after innoculation with the powdery mildew fungus Golovinomyces cichoracearum. After innoculation with the pathogenic bac-terium Pseudomonas syringae pv. tomato, the baceria in the leaves of transgenic plants was significantly less than the wild-type plants, indicating that the transgenic plants displayed enhanced disease resistance to pathogens. These results demonstrate a potential use of Ponericin W1 in genetic engineering for broad-spectrum plant disease resistance.

Key words: Ponericin W1, transgenic, antimicrobial peptides, plant pathogen

CHEN Yong-Fang SUN Peng-Wei TANG Ding-Zhong. Antimicrobial activities of ant Ponericin W1 against plant pathogens in vitro and the disease resistance in its transgenic Arabidopsis[J]. HEREDITAS, 2013, 35(8): 1023-1029.

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Antimicrobial activities of ant Ponericin W1 against plant pathogens in vitro and the disease resistance in its transgenic Arabidopsis

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