毛竹MYB转录因子PeMYB2的克隆与功能分析

doi:10.3724/SP.J.1005.2013.01217

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HEREDITAS ›› 2013, Vol. 35 ›› Issue (10): 1217-1225.doi: 10.3724/SP.J.1005.2013.01217

Cloning and functional analysis of Phyllostachys edulis MYB tran-scription factor PeMYB2

State Key Laboratory Cultivation Base of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin’an 311300, China

State Key Laboratory Cultivation Base of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin’an 311300, China

Received:2013-04-23 Revised:2013-06-04 Online:2013-10-20 Published:2013-10-25

Abstract

Abstract:

MYB-type transcription factor is one of the largest families in plants, which plays important roles in accepting stress signals from environment and regulating the expression of stress-tolerant genes. In this paper, using homologous cloning and RACE technology, a MYB-type transcription factor, designated PeMYB2, was cloned from Phyllostachys edulis. The results of bioinformatics showed that PeMYB2 is a typical R2R3-MYB. It contained two tandem repeats in its N-terminus, and a membrane protein DUF3651 in its C-terminus. In addition, phylogenetic analysis indicated that PeMYB2 shared the highest homology with 85.98% to OsMYB18 protein from Oryza sativa spp. Japonica. In addition, a yeast one-hybrid assay showed that PeMYB2 could activate the expression of downstream genes. After PeMYB2 was transformed into Arabidopsis thaliana, seven PeMYB2 transgenic Arabidopsis lines were obtained. Phenotypic analysis of the transgenic and wild-type Arabidopsis showed that over-expression of PeMYB2 caused delayed flower or dwarfism in transgenic Arabidopsis. Under the abiotic stress conditions, such as salt and cold stresses, the over-expression of PeMYB2 in Arabidopsis had higher survival rate than the wild-type Arabidopsis. Expression analysis of saline stress response marker genes in the transgenic and wild-type plants under the salt stress condition showed that PeMYB2 regulated the expression of NXH1, SOS1, RD29A, and COR15A. As the result, PeMYB2 might play an important role in various responses to abiotic stresses in P. edulis.

Key words: Phyllostachys edulis, PeMYB2, abiotic stress, transgene

XIAO Dong-Chang ZHANG Zhi-Jun XU Ying-Wu YANG Li ZHANG Feng-Xue. Cloning and functional analysis of Phyllostachys edulis MYB tran-scription factor PeMYB2[J]. HEREDITAS, 2013, 35(10): 1217-1225.

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Cloning and functional analysis of Phyllostachys edulis MYB tran-scription factor PeMYB2

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