北京汉族群体30个常染色体InDel位点群体遗传学及法医学研究

doi:10.3724/SP.J.1005.2013.01368

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HEREDITAS ›› 2013, Vol. 35 ›› Issue (12): 1368-1376.doi: 10.3724/SP.J.1005.2013.01368

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Genetic polymorphism and forensic application of 30 InDel loci of Han population in Beijing

BAI Ru-Feng, JIANG Li-Zhe, ZHANG Zhong, SHI Mei-Sen

Key Laboratory of Evidence Science, Ministry of Education, China University of Political Science and Law, Beijing 100040, China

Received:2013-07-16 Revised:2013-09-27 Online:2013-12-20 Published:2013-11-25

Abstract

Abstract:

To study the genetic diversities of 30 insertion-deletion (InDel) polymorphisms loci of Han population in Beijing, and to evaluate their forensic application, 210 unrelated healthy individuals of Han population in Beijing were investigated to determine the distributions of allele frequencies by using Investigator® DIP system. The PCR products were detected with ABI 3130 XL Genetic Analyzer. Forensic parameters were calculated with relevant statistical analysis software. As a result, after the Bonferroni correction at a 95% significance level, there were no significant departures from Hardy-Weinberg equilibrium or significant linkage disequilibrium between the loci. The power of discrimination (DP) varies between 0.2690 (HLD118) and 0.6330 (HLD45), and the combined discrimination power (TDP) for the 30 InDel loci is 0.999999999985. The combined power of exclusion was 0.98771049 in trio cases (CPE_trio) and 0.94579456 in duo cases (CPEduo). The parentage testing of 32 cases revealed no mutations happened to 30 InDel loci. Multiplex detection of the 30 InDel loci revealed a highly polymorphic genetic distribution in Beijing Han population, which represents a complementary tool in human identification studies, especially in challenging DNA cases.

Key words: insertion and deletion polymorphisms(InDel), Investigator®, DIPplex, genetic diversity, forensic science

BAI Ru-Feng, JIANG Li-Zhe, ZHANG Zhong, SHI Mei-Sen. Genetic polymorphism and forensic application of 30 InDel loci of Han population in Beijing[J]. HEREDITAS, 2013, 35(12): 1368-1376.

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Genetic polymorphism and forensic application of 30 InDel loci of Han population in Beijing

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