生物法合成伤寒O-糖蛋白结合疫苗及其免疫原性评估

doi:10.16288/j.yczz.15-001

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HEREDITAS(Beijing) ›› 2015, Vol. 37 ›› Issue (5): 473-479.doi: 10.16288/j.yczz.15-001

• Research Articles • Previous Articles Next Articles

Preparation and immunogenicity-evaluation of typhoid O-specific polysaccharides bio-conjugate vaccines

Zhehui Peng^{1, 2}, Chao Pan², Peng Sun², Erling Feng², Jun Wu², Li Zhu², Qingzhong Peng¹, Hengliang Wang²

1. College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China;
2. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China

Received:2015-01-25 Online:2015-05-20 Published:2015-05-20

Abstract

Abstract: Typhoid fever caused by Salmonella Typhi is still a major public health problem in developing countries. In this study, we constructed a genetically modified Salmonella Typhi strain expressing O-specific polysaccharides (OPS) antigen conjugated to a carrier, recombinant Pseudomonas aeruginosa exotoxin A(rEPA_N29). The conjugates (OPS-rEPA_N29) were further purified and evaluated for their immunogenicity. The results of ELISA showed that the conjugates evoked higher titers of IgG than OPS, suggesting that rEPA_N29 increased immunogenicity of OPS significantly as a carrier. Moreover, three injections with 3-week interval evoked slightly higher titers of IgG than three injections with 2-week interval. However, injection of excess conjugates could not evoke higher titers of IgG against lipid polysaccharide (LPS). In summary, our study provides a new strategy for preparing polysaccharides-protein conjugate vaccines as well as similar bio-conjugate vaccines of other Gram-negative pathogens.

Key words: conjugate vaccine, Salmonella Typhi, synthetic biology, rEPA, O-polysaccharide antigen

Zhehui Peng, Chao Pan, Peng Sun, Erling Feng, Jun Wu, Li Zhu, Qingzhong Peng, Hengliang Wang. Preparation and immunogenicity-evaluation of typhoid O-specific polysaccharides bio-conjugate vaccines[J]. HEREDITAS(Beijing), 2015, 37(5): 473-479.

References

[1] 2013年卫生统计提要, 卫生部网站: www.moh.gov.cn/ ewebeditor/uploadfile/2014/04/20140430131845405.pdf.
[2] Bhutta ZA. Impact of age and drug resistance on mortality in typhoid fever. Arch Dis Child , 1996, 75(3): 214-217.
[3] Lerouge I, Vanderleyden J. O-antigen structural variation: mechanisms and possible roles in animal/plant-microbe interactions. FEMS Microbio Rev , 2002, 26(1): 17-47.
[4] Faridmoayer A, Fentabil MA, Mills DC, Klassen JS, Feldman MF. Functional characterization of bacterial oligosaccharyltransferases involved in O-linked protein glycosylation. J Bacteriol , 2007, 189(22): 8088-8098.
[5] Hug I, Feldman MF. Analogies and homologies in lipopolysaccharide and glycoprotein biosynthesis in bacteria. Glycobiology , 2011, 21(2): 138-151.
[6] 王恒樑, 冯尔玲, 史兆兴, 姚潇, 苏国富, 黄留玉. 用Red系统快速敲除痢疾杆菌asd基因. 军事医学科学院院刊, 2002, 26(3): 172-175.
[7] Acosta CJ, Yang HH, Ning W, Qion G, Qun D, Ma XL, Zhou BD, Liu W, Carolina Danovaro-Holliday M, Leon Ochiai R, Wang XY, Kim DR, Xu ZY, Dong BQ, Galindo CM, Clemens JD. Efficacy of a locally produced, Chinese Vi polysaccharide typhoid fever vaccine during six years of follow-up. Vaccine , 2005, 23(48-49): 5618-5623.
[8] Szu SC, Stone AL, Robbins JD, Schneerson R, Robbins JB. Vi capsular polysaccharide-protein conjugates for prevention of typhoid fever. Preparation, characterization, and immunogenicity in laboratory animals. J Exper Med , 1987, 166(5): 1510-1524.
[9] Jones C. Vaccines based on the cell surface carbohydrates of pathogenic bacteria. An Acad Bras Cienc , 2005, 77(2): 293-324.
[10] Terra VS, Mills DC, Yates LE, Abouelhadid S, Cuccui J, Wren BW. Recent developments in bacterial protein glycan coupling technology and glycoconjugate vaccine design. J Med Microbiol , 2012, 61(Pt7): 919-926.
[11] Dagan R, Poolman J, Siegrist CA. Glycoconjugate vaccines and immune interference: A review. Vaccine , 2010, 28(34): 5513-5523.
[12] Passwell JH, Harlev E, Ashkenazi S, Chu C, Miron D, Ramon R, Farzan N, Shiloach J, Bryla DA, Majadly F, Roberson R, Robbins JB, Schneerson R. Safety and immunogenicity of improved Shigella O-specific polysaccharide-protein conjugate vaccines in adults in Israel. Infect Immun , 2001, 69(3): 1351-1357.
[13] Ahmed A, Li JP, Shiloach Y, Robbins JB, Szu SC. Safety and immunogenicity of Escherichia coli O157 O-specific polysaccharide conjugate vaccine in 2-5-year-old children. J Infect Dis , 2006, 193(4): 515-521.
[14] Ihssen J, Kowarik M, Dilettoso S, Tanner C, Wacker M, Thöny-Meyer L. Production of glycoprotein vaccines in Escherichia coli . Microb Cell Fac , 2010, 61: 1-13.
[15] Fernandez S, Palmer DR, Simmons M, Sun PF, Bisbing J, McClain S, Mani S, Burgess T, Gunther V, Sun W. Potential role for toll-like receptor 4 in mediating Escherichia coli maltose-binding protein activation of dendritic cells. Infect Immun , 2007, 75(3): 1359-1363.
[16] Paoletti LC, Kasper DL, Michon F, DiFabio J, Jennings HJ, Tosteson TD, Wessels MR. Effects of chain length on the immunogenicity in rabbits of group B streptococcus type III oligosaccharide-tetanus toxoid conjugates. J Clin Invest , 1992, 89(1): 203-209.

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Preparation and immunogenicity-evaluation of typhoid O-specific polysaccharides bio-conjugate vaccines

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