欧洲千里光SvAPETALA1-5基因对花器官发育的影响

doi:10.16288/j.yczz.24-147

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Hereditas(Beijing) ›› 2024, Vol. 46 ›› Issue (9): 727-736.doi: 10.16288/j.yczz.24-147

• Research Article • Previous Articles Next Articles

Effects of SvAPETALA1-5 gene on floral organ development in Senecio vulgaris

Yuna Zhang(), Yanmin Hao, Minlong Cui, Chunlan Piao()

Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable,Ministry of Agriculture and Rural Affairs, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticultural Science, Zhejiang A & F University, Hangzhou 311300, China

Received:2024-05-23 Revised:2024-07-17 Online:2024-09-20 Published:2024-08-01
Contact: Chunlan Piao E-mail:1047925191@qq.com;2488335116@qq.com
Supported by:
Cooperative R&D Project of Zhejiang Academy of Agricultural Sciences(H20220312)

Abstract

Abstract:

Asteraceae is a large class of eudicots with complex capitulum, and little is known regarding the molecular regulation mechanism of flower development. APETALA1(AP1) belongs to the MADS-box gene family and plays a key role in plant floral induction and floral organ development. In this study, the bioinformatics and tissue-specific expression of AP1 homologous gene SvAP1-5 in Senecio vulgaris were analyzed. Based on VIGS technology, SvAP1-5 gene silencing plants were created, and SvAP1-5 was overexpressed in Solanum nigrum. The results of bioinformatics analysis showed that SvAP1-5 gene had typical MADS-box and K-box structure, and contains FUL motif and paleoAP1 motif at the C-terminal. SvAP1-5 belongs to the euFUL branch of AP1 gene. qRT-PCR results showed that SvAP1-5 was expressed in bracts, petals and carpels, and was highly expressed in carpels. Compared with the control group, SvAP1-5 gene silencing resulted in irregular petal dehiscence, increased stigma division, and carpel dysplasia. The fruit development of SvAP1-5 overexpressing S.nigrum plants was abnormal, and the hyperplastic tissue similar to fruit appeared. In summary, SvAP1-5 gene may be involved in the development of petals and carpels and plays an important role during the development of S.vulgaris.

Key words: Senecio vulgaris, SvAPETALA1-5, flower development, functional research

Yuna Zhang, Yanmin Hao, Minlong Cui, Chunlan Piao. Effects of SvAPETALA1-5 gene on floral organ development in Senecio vulgaris[J]. Hereditas(Beijing), 2024, 46(9): 727-736.

Figures/Tables 7

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引物名称	引物序列(5′→3′)	用途
TRV2::SvAP1-5	F：AACTCGAGCAATCTCTCAGCTCCAA	VIGS载体构建
TRV2::SvAP1-5	R：AAGGATCCGATGAATCATCCAAGGAGGC	VIGS载体构建
qSvAP1-5	F：GTTGTGTGATGCTGACGTGG	qRT-PCR引物
qSvAP1-5	R：GCGTGTTCCAGAGTCCAGTT	qRT-PCR引物
Sv18S	F：CGCGCGCTACACTGATGTATTCAA	内参基因
Sv18S	R：TACAAAGGGCAGGGACGTAGTCAA	内参基因

Effects of SvAPETALA1-5 gene on floral organ development in Senecio vulgaris

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[1]	Feifei Li, Yanmin Hao, Minlong Cui, Chunlan Piao. Cloning and functional analysis of RADIALIS-like 1 gene from Antirrhinum majus [J]. Hereditas(Beijing), 2023, 45(6): 526-535.
[2]	Sinan Luan, Lele Liu, Jiayuan Zhou, Nurasya Imam, Minlong Cui, Chunlan Piao. Functional analysis of flower development related gene SvGLOBOSA from Senecio vulgaris [J]. Hereditas(Beijing), 2022, 44(6): 521-530.
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