金鱼草RADIALIS-like 1基因克隆与功能研究

doi:10.16288/j.yczz.23-057

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Hereditas(Beijing) ›› 2023, Vol. 45 ›› Issue (6): 526-535.doi: 10.16288/j.yczz.23-057

• Research Article • Previous Articles Next Articles

Cloning and functional analysis of RADIALIS-like 1 gene from Antirrhinum majus

Feifei Li(), Yanmin Hao, Minlong Cui, Chunlan Piao()

Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, China

Received:2023-03-14 Revised:2023-04-11 Online:2023-06-20 Published:2023-05-29
Contact: Piao Chunlan E-mail:1399745127@qq.com;2488335116@qq.com
Supported by:
Cooperative R & D Project of Zhejiang Academy of Agricultural Sciences(H20220312)

Abstract

Abstract:

MYB is one of the largest transcription factor families in plants. Among them, the R3-MYB transcription factor RADIALIS (RAD) plays a very important role in the flowers development in Antirrhinum majus. In this study, a R3-MYB gene similar to RAD was found by analyzing the genome of A. majus, which was named AmRADIALIS-like 1 (AmRADL1). The gene function was predicted through bioinformatics. The relative expression levels in different tissues and organs of wild-type A. majus were analyzed by qRT-PCR. AmRADL1 was overexpressed in A. majus, and the transgenic plants were analyzed by morphological observation and histological staining. The results showed that the open reading frame (ORF) of AmRADL1 gene was 306 bp in length, encoding 101 amino acids. It has typical SANT domain, and the C-terminal contains a CREB motif, which was highly homologous to tomato SlFSM1. The results of qRT-PCR showed that AmRADL1 was expressed in roots, stems, leaves and flowers, and the expression level was higher in flowers. Further analysis of its expression in different floral organs showed that AmRADL1 had the highest expression in carpel. The results of histological staining analysis of the transgenic plants showed that compared with the wild type, although the size of the carpel cells of the transgenic plants did not change significantly, the placenta area in the carpel became smaller and the number of cell decreased. In summary, AmRADL1 may be involved in the regulation of carpel development, but the specific mechanism of action in carpel remains to be further studied.

Key words: Antirrhinum majus, RADIALIS-like gene, flower development, expression analysis, histological stain

Feifei Li, Yanmin Hao, Minlong Cui, Chunlan Piao. Cloning and functional analysis of RADIALIS-like 1 gene from Antirrhinum majus[J]. Hereditas(Beijing), 2023, 45(6): 526-535.

Figures/Tables 8

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引物名称	引物序列(5′→3′)	用途
AmUBI	F: ATTGGTGCTGAGGTTGAGA	内参基因
AmUBI	R: ACAACTGACTCCAGCAAACG	内参基因
Kan	F: AGATGGATTGCACGCAGGTTC	Kan引物鉴定
Kan	R: GTGGTCGAATGGGCAGGTAG	Kan引物鉴定
AmRADL1	F: AAGGATCCATGGCGTCGAGTTCGTTGTCAC	基因克隆及荧光定量PCR引物
AmRADL1	R: AAGAGCTCTTAGCCACTAAAATAAATAAAAACAT	基因克隆及荧光定量PCR引物

Cloning and functional analysis of RADIALIS-like 1 gene from Antirrhinum majus

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