关键词: 遗传学, 实验教学, 转基因, 黑腹果蝇

Abstract: Integrating frontline research into undergraduate laboratory classes is an effective strategy to improve teaching quality, stimulate students’ research interest, and enhance their scientific knowledge and innovative capabilities. Transgenic animal construction technology involves multiple genetic principles; designing it as an experimental teaching module helps students deepen their understanding of genetic information transmission and gene function. Transgenic experimental systems using vertebrates are constrained by high costs and long experimental periods, whereas Drosophila-based transgenic animal experiments are feasible for teaching and can be integrated with current research frontiers. Here, we designed a comprehensive genetics experiment module on transgenic Drosophila, which includes four progressive steps: molecular cloning, microinjection, genetic screening, and fluorescence microscopy. Through theoretical instruction on transgenic expression plasmids, chimeras, germline transmission, and other genetic concepts, combined with experimental operations including microinjection, cross-based screening, and phenotypic observation, as well as extended discussions on transgenic mammalian procedures, this scheme effectively cultivates students’ problem-solving abilities and innovation.

Key words:  , genetics, experimental teaching, transgene, Drosophila melanogaster