利用椭圆小球藻硝酸还原酶缺失突变体为生物反应器表达兔防御素NP-1蛋白

张小宇，王鹏，赵世民，李霞，沈昕，孙勇如，储成才，王义琴

doi:10.1360/yc-006-1580

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2006 , Vol. 28 >Issue 12: 1580 - 1580~1584

DOI: https://doi.org/10.1360/yc-006-1580

研究报告

利用椭圆小球藻硝酸还原酶缺失突变体为生物反应器表达兔防御素NP-1蛋白

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(1. 中国科学院遗传与发育生物学研究所, 北京 100101; 2. 北京林业大学生物科学与技术学院, 北京 100083)

收稿日期: 2006-03-10

修回日期: 2006-05-12

网络出版日期: 2006-12-10

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Expression of Rabbit Neutrophile Peptide-1 in Nitrate Reductase- deficient Mutant of Chlorella ellipsoidea

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(1. State Key Laboratory of Plant Genomics and National Plant Gene Research Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 2. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China)

Received date: 2006-03-10

Revised date: 2006-05-12

Online published: 2006-12-10

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摘要

利用转基因小球藻为生物反应器生产兔防御素NP-1蛋白具有重要的应用价值。本研究利用椭圆小球藻(Chlorella ellipsoidea)硝酸还原酶(nitrate reductase)缺失突变体为受体, 构建了包含NPTII基因和硝酸还原酶基因两个筛选标记的兔防御素蛋白表达载体, 采用电激法将目的基因转入椭圆小球藻硝酸还原酶缺失突变体nrm-4, 获得了正确表达防御素蛋白的转基因藻, 从而表明通过硝酸还原酶作为筛选标记基因并结合硝酸还原酶缺失突变体可作为较好的小球藻生物反应器生产模式。

关键词： 椭圆小球藻; 硝酸还原酶缺失突变体; 兔防御素

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张小宇，王鹏，赵世民，李霞，沈昕，孙勇如，储成才，王义琴 . 利用椭圆小球藻硝酸还原酶缺失突变体为生物反应器表达兔防御素NP-1蛋白[J]. 遗传, 2006 , 28(12) : 1580 -1580~1584 . DOI: 10.1360/yc-006-1580

Abstract

Application of transgenic Cholrella as bioreactor to express rabbit neutrophile pepetide-1 (NP-1) shows great practical value. In this paper, an NP-1 expression vector containing two selective marker genes NPTII and nitrate reductase gene was constructed. The NP-1 gene was transformed into the nitrate reductase-deficient mutant nrm-4 of Chlorella ellipsoidea via electroporation, and the transgenic alga expressed the active NP-1 were obtained.

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