磷酸化修饰是一种重要的蛋白质化学修饰, 对蛋白质功能的完成或改变起到重要作用。该领域的研究存在很多技术难点, 对该领域研究形成了挑战。近年来相关技术有了很多突破, 磷酸化研究也取得了很多新的成就。文章将从磷酸化蛋白的检出、磷酸化蛋白质和肽段的富集、生物质谱技术的改进以及磷酸化蛋白和多肽的定量与比较几个方面介绍该研究领域的技术进展。

Phosphorylation is an important protein chemical modification which plays vital roles in completion and change of protein function. There are lots of technique difficulties in this field which become the challenges of the research. In recent years, phosphorylation research has made many novel achievements profit from the breakthroughs of cross-correlation techniques. In this article, we introduce the technique development of this research field from many aspects such as detection of phosphorylated proteins, enrichment of phosphorylated proteins and peptides, improvement of biological mass spec-trometry-based techniques, quantification and comparison of phosphorylated proteins and peptides.