以90日龄草科鸡为实验材料, 对H-FABP基因进行PCR扩增, 采用PCR-SSCP技术结合测序分析了H-FABP基因在草科鸡中的多态性。结果表明: 草科鸡中存在332 G→A、534 G→A、835 C→T、1131→A、1294C→A、2329C→T、2372C→T、2636C→T等8处突变。经过基因型与屠宰性能的关联分析得知: 在7对产生多态的引物中, 只有第1对引物的各基因型与活重、屠体重、胸肌重、腿肌重和半净膛重差异显著(P<0.05); 全净膛重在不同基因型间差异极显著(P<0.01)。 由此推测, H-FABP可能对于鸡屠宰性能具有很大的影响或与控制屠宰性能的主基因连锁。

In this experiment, Caoke chicken was used to analyze H-FABP gene polymorphism by PCR-SSCP. Results indicated that there were eight mutation sites in Caoke chicken: 332G→A, 534G→A, 835C→T, 1131→A, 1294C→A, 2329C→T, 2372C→T, 2636C→T. In seven pairs of primers that generated polymorphisms, only the first pair resulted in genotypes that showed a significant difference in body weight, carcass weight, breast weight, leg weight and half-eviscerated weight (P < 0.05). It implied that H-FABP could have a major effect on slaughter performance or could be linked to gene(s) that significantly affect slaughter performance in chicken.