随着蛋白质组学概念的提出以及诸如血浆蛋白质组等有影响力的计划开展, 蛋白质组研究迅速发展起来, 这门基于分析化学和物理化学的领域也逐渐为广大生物学家所关注, 同时也相应地在细胞生物学、生物化学等领域的研究中崭露头角。蛋白质表达量的变化以及各种各样的修饰无不反映出机体对环境变化的应激和自身功能的需要。因此, 定量蛋白质组和修饰化的蛋白质组成为了目前蛋白质组研究的重要领域之一。文章着重从采用化学标记实现定量和修饰化研究这个角度来介绍近些年来在这方面取得的进展, 希望对生物学领域的研究有所借鉴。

With the proteomics concept introduction and effective projects implement as Human Plasma Project, the proteomics research is being developed rapidly, which is based on the analytical chemistry and physical chemistry. More and more biologists paid attention to this field, and got some important results in cell biology and biochemistry. The variation of protein quantity and various modifications in the cell reflects the environment stress and the function requirement. To inves-tigate these variations, proteomic technology provided powerful tools. In this article, chemical probe in quantitative and modification study is discussed in details.